Search: id:"swepub:oai:DiVA.org:umu-215721" >
Experimental phasin...
Experimental phasing opportunities for macromolecular crystallography at very long wavelengths
-
- El Omari, Kamel (author)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
-
- Duman, Ramona (author)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
-
- Mykhaylyk, Vitaliy (author)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
-
show more...
-
- Orr, Christian M. (author)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
-
- Latimer-Smith, Merlyn (author)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom
-
- Winter, Graeme (author)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom
-
- Grama, Vinay (author)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom
-
- Qu, Feng (author)
- Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom; Department of Life Sciences, Imperial College London, London, United Kingdom; Department of Biochemistry, University of Oxford, Oxford, United Kingdom
-
- Bountra, Kiran (author)
- Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom; Department of Life Sciences, Imperial College London, London, United Kingdom
-
- Kwong, Hok Sau (author)
- Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom; Department of Life Sciences, Imperial College London, London, United Kingdom
-
- Romano, Maria (author)
- Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom; Department of Life Sciences, Imperial College London, London, United Kingdom; Institute of Biostructures and Bioimaging, IBB, CNR, Naples, Italy; Department of Pharmacy, University of Naples “Federico II”, Naples, Italy
-
- Reis, Rosana I. (author)
- National Physical Laboratory, Hampton Road, Teddington, United Kingdom
-
- Vogeley, Lutz (author)
- Charles River Discovery Research Services UK, Chesterford Research Park, Saffron Walden, United Kingdom; Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany
-
- Vecchia, Luca (author)
- Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
-
- Owen, C. David (author)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
-
- Wittmann, Sina (author)
- Department of Biochemistry, University of Oxford, Oxford, United Kingdom; Institute of Molecular Biology (IMB), Ackermannweg 4, Mainz, Germany
-
- Renner, Max (author)
- Umeå universitet,Kemiska institutionen,Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
-
- Senda, Miki (author)
- Structural Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Ibaraki, Tsukuba, Japan
-
- Matsugaki, Naohiro (author)
- Structural Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Ibaraki, Tsukuba, Japan; Department of Materials Structure Science, School of High Energy Accelerator Science, The Graduate University of Advanced Studies (Soken-dai), 1-1 Oho, Ibaraki, Tsukuba, Japan
-
- Kawano, Yoshiaki (author)
- Advanced Photon Technology Division, RIKEN SPring-8 Center, Hyogo, Japan
-
- Bowden, Thomas A. (author)
- Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
-
- Moraes, Isabel (author)
- National Physical Laboratory, Hampton Road, Teddington, United Kingdom
-
- Grimes, Jonathan M. (author)
- Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
-
- Mancini, Erika J. (author)
- School of Life Sciences, University of Sussex, Falmer, Brighton, United Kingdom
-
- Walsh, Martin A. (author)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
-
- Guzzo, Cristiane R. (author)
- Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
-
- Owens, Raymond J. (author)
- Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom; The Rosalind Franklin Institute, Harwell Campus, Oxford, United Kingdom
-
- Jones, E. Yvonne (author)
- Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
-
- Brown, David G. (author)
- Charles River Discovery Research Services UK, Chesterford Research Park, Saffron Walden, United Kingdom
-
- Stuart, Dave I. (author)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
-
- Beis, Konstantinos (author)
- Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom; Department of Life Sciences, Imperial College London, London, United Kingdom
-
- Wagner, Armin (author)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
-
show less...
-
(creator_code:org_t)
- Nature Publishing Group, 2023
- 2023
- English.
-
In: Communications Chemistry. - : Nature Publishing Group. - 2399-3669. ; 6:1
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://umu.diva-por... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Despite recent advances in cryo-electron microscopy and artificial intelligence-based model predictions, a significant fraction of structure determinations by macromolecular crystallography still requires experimental phasing, usually by means of single-wavelength anomalous diffraction (SAD) techniques. Most synchrotron beamlines provide highly brilliant beams of X-rays of between 0.7 and 2 Å wavelength. Use of longer wavelengths to access the absorption edges of biologically important lighter atoms such as calcium, potassium, chlorine, sulfur and phosphorus for native-SAD phasing is attractive but technically highly challenging. The long-wavelength beamline I23 at Diamond Light Source overcomes these limitations and extends the accessible wavelength range to λ = 5.9 Å. Here we report 22 macromolecular structures solved in this extended wavelength range, using anomalous scattering from a range of elements which demonstrate the routine feasibility of lighter atom phasing. We suggest that, in light of its advantages, long-wavelength crystallography is a compelling option for experimental phasing.
Subject headings
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
El Omari, Kamel
-
Duman, Ramona
-
Mykhaylyk, Vital ...
-
Orr, Christian M ...
-
Latimer-Smith, M ...
-
Winter, Graeme
-
show more...
-
Grama, Vinay
-
Qu, Feng
-
Bountra, Kiran
-
Kwong, Hok Sau
-
Romano, Maria
-
Reis, Rosana I.
-
Vogeley, Lutz
-
Vecchia, Luca
-
Owen, C. David
-
Wittmann, Sina
-
Renner, Max
-
Senda, Miki
-
Matsugaki, Naohi ...
-
Kawano, Yoshiaki
-
Bowden, Thomas A ...
-
Moraes, Isabel
-
Grimes, Jonathan ...
-
Mancini, Erika J ...
-
Walsh, Martin A.
-
Guzzo, Cristiane ...
-
Owens, Raymond J ...
-
Jones, E. Yvonne
-
Brown, David G.
-
Stuart, Dave I.
-
Beis, Konstantin ...
-
Wagner, Armin
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Physical Science ...
-
and Atom and Molecul ...
- Articles in the publication
-
Communications C ...
- By the university
-
Umeå University