T lymphocyte expression of thrombospondin-1 and adhesion to extracellular matrix components.

Ivanoff, Anna (author): Karolinska Institutet,Umeå universitet,Klinisk immunologi

Bergström, Sten-Erik (author)

van Nerven, Joost (author)

Holgersson, Jan (author)

Sundqvist, Karl-Gösta (author): Karolinska Institutet,Umeå universitet,Klinisk immunologi

(creator_code:org_t)

2002
2002
English.
In: European Journal of Immunology. - 0014-2980 .- 1521-4141. ; 32:4, s. 1069-1079

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; http://kipublication...; show less...

Abstract Subject headings

The mechanisms controlling the formation of pseudopodia and other active cell edges in T lymphocytes are not understood. We show here that T lymphocytes express thrombospondin-1 (TSP-1). TSP-1 in T lymphocytes has a high turnover as shown by the fact that brefeldin and monensin rapidly increase while cycloheximide tend to decrease the cellular TSP-1 content. T cell TSP-1 is preferentially stored intracellularly and shows variable cell surface expression. T lymphocyte adhesion to fibronectin and collagen type IV induces TSP-1 expression on the cell surface via a brefeldin sensitive mechanism. A monoclonal antibody to TSP-1 inhibits the flattening and pseudopodia formation of the adherent T cells. Furthermore, the same antibody to TSP-1 also exerts an inhibitory effect on T cell migration in the absence of exogenous TSP-1. These results indicate that endogenous TSP-1 is part of an adhesion-dependent mechanism controlling cytoplasmic spreading and migration in T lymphocytes.

By the author/editor: Li, Shu Shun; Ivanoff, Anna; Bergström, Sten- ...; Sandström, Ander ...; Christensson, Bi ...; van Nerven, Joos ...; show more...; Holgersson, Jan; Hauzenberger, Da ...; Arencibia, Ignac ...; Sundqvist, Karl- ...; show less...

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