Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosis

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Fältnamn	Indikatorer	Metadata
000		03080naa a2200433 4500
001		oai:DiVA.org:umu-43033
003		SwePub
008		110415s2006 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:prod.swepub.kib.ki.se:1937200
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-430332 URI
024	7	a https://doi.org/10.1111/j.1469-0691.2005.01332.x2 DOI
024	7	a http://kipublications.ki.se/Default.aspx?queryparsed=id:19372002 URI
040		a (SwePub)umud (SwePub)ki
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Edvinsson, B4 aut
245	1 0	a Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosis
264	1	b Elsevier BV,c 2006
338		a print2 rdacarrier
520		a Sensitive and rapid detection of infection with Toxoplasma gondii in transplanted immunocompromised patients is crucial for a good prognosis. Two DNA fragments are used currently for detecting T. gondii infection by PCR, i.e., the B1 gene and a 529-bp repeat element that exists in 200-300 copies/genome. This study investigated whether targeting the 529-bp repeat element gives better sensitivity and accuracy than can be obtained when targeting the B1 gene (35 copies) when concentrations of T. gondii DNA are low. The results demonstrated that detection of the 529-bp repeat element increased diagnostic sensitivity and accuracy. Addition of an internal amplification control did not affect the PCR performance and was useful in order to monitor PCR inhibition by non-specific DNA in the LightCycler instrument. The real-time PCR was used successfully in a clinical context to monitor parasitaemia in the blood of a transplant recipient suffering from toxoplasmosis.
653		a B1 gene; diagnosis; internal amplification control; real-time PCR; repeat element; toxoplasmosis
700	1	a Lappalainen, M4 aut
700	1	a Evengård, Birgitta,d 1952-u Karolinska Institutet, Department of Laboratory Medicine, Division of Clinical Bacteriology, Karolinska University Hospital Huddinge, Stockholm, Sweden,Publikation Karolinska Institutet4 aut0 (Swepub:umu)biev0002
700	1	a Buffalano, W4 aut
700	1	a Ferguson, D4 aut
700	1	a Guy, E4 aut
700	1	a Jenum, P4 aut
700	1	a Nowakowska, D4 aut
700	1	a Pelloux, H4 aut
700	1	a Stray-Pedersen, B4 aut
700	1	a Szénási, Z4 aut
710	2	a Karolinska Institutet, Department of Laboratory Medicine, Division of Clinical Bacteriology, Karolinska University Hospital Huddinge, Stockholm, Swedenb Publikation Karolinska Institutet4 org
773	0	t Clinical Microbiology and Infectiond : Elsevier BVg 12:2, s. 131-136q 12:2<131-136x 1198-743Xx 1469-0691
856	4	u http://www.clinicalmicrobiologyandinfection.com/article/S1198743X14633966/pdf
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-43033
856	4 8	u https://doi.org/10.1111/j.1469-0691.2005.01332.x
856	4 8	u http://kipublications.ki.se/Default.aspx?queryparsed=id:1937200

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Av författaren/redakt...: Edvinsson, B; Lappalainen, M; Evengård, Birgit ...; Buffalano, W; Ferguson, D; Guy, E; visa fler...; Jenum, P; Nowakowska, D; Pelloux, H; Stray-Pedersen, ...; Szénási, Z; visa färre...

Artiklar i publikationen: Clinical Microbi ...

Av lärosätet: Umeå universitet; Karolinska Institutet

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