id:"swepub:oai:DiVA.org:umu-48655"
Search: id:"swepub:oai:DiVA.org:umu-48655" > P13, an integral me...
- 1 of 1
- Previous record
- Next record
- To hitlist
P13, an integral membrane protein of Borrelia burgdorferi, is C-terminally processed and contains surface-exposed domains
-
- Noppa, Laila (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi
-
- Östberg, Yngve (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
-
- Lavrinovicha, Marija (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi
- show more...
- show less...
- (creator_code:org_t)
- 2001
- 2001
- English.
- In: Infection and Immunity. - 0019-9567 .- 1098-5522. ; 69:5, s. 3323-3334
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- To elucidate antigens present on the bacterial surface of Borrelia burgdorferi sensu lato that may be involved in pathogenesis, we characterized a protein, P13, with an apparent molecular mass of 13 kDa. The protein was immunogenic and was expressed in large amounts during in vitro cultivation compared to other known antigens. An immunofluorescence assay, immunoelectron microscopy, and protease sensitivity assays indicated that P13 is surface exposed. The deduced sequence of the P13 peptide revealed a possible signal peptidase type I cleavage site, and computer analysis predicted that P13 is an integral membrane protein with three transmembrane-spanning domains. Mass spectrometry, in vitro translation, and N- and C-terminal amino acid sequencing analyses indicated that P13 was posttranslationally processed at both ends and modified by an unknown mechanism. Furthermore, p13 belongs to a gene family with five additional members in B. burgdorferi sensu stricto. The p13 gene is located on the linear chromosome of the bacterium, in contrast to five paralogous genes, which are located on extrachromosomal plasmids. The size of the p13 transcript was consistent with a monocistronic transcript. This new gene family may be involved in functions that are specific for this spirochete and its pathogenesis.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Infection and Immunity (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Noppa, Laila
- Östberg, Yngve
- Lavrinovicha, Ma ...
- Bergström, Sven
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Cell and Molecul ...
- Articles in the publication
- Infection and Im ...
- By the university
- Umeå University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
