id:"swepub:oai:DiVA.org:umu-59880"
Search: id:"swepub:oai:DiVA.org:umu-59880" > Purification of tra...
- 1 of 1
- Previous record
- Next record
- To hitlist
Purification of transfer RNA (m5U54)-methyltransferase from Escherichia coli. Association with RNA.
-
- Ny, Tor (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
- Lindström, H R (author)
- Hagervall, T G (author)
- show more...
- Björk, G R (author)
- show less...
- (creator_code:org_t)
- 1988
- 1988
- English.
- In: European Journal of Biochemistry. - 0014-2956 .- 1432-1033. ; 177:3, s. 467-75
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- tRNA (m5U54)-methyltransferase (EC 2.1.1.35) catalyzes the transfer of methyl groups from S-adenosyl-L-methionine to transfer ribonucleic acid (tRNA) and thereby forming 5-methyluridine (m5U, ribosylthymine) in position 54 of tRNA. This enzyme, which is involved in the biosynthesis of all tRNA chains in Escherichia coli, was purified 5800-fold. A hybrid plasmid carrying trmA, the structural gene for tRNA (m5U54)-methyltransferase was used to amplify genetically the production of this enzyme 40-fold. The purest fraction contained three polypeptides of 42 kDa, 41 kDa and 32 kDa and a heterogeneous 48-57-kDa RNA-protein complex. All the polypeptides seem to be related to the 42/41-kDa polypeptides previously identified as the tRNA (m5U54)-methyltransferase. RNA comprises about 50% (by mass) of the complex. The RNA seems not to be essential for the methylation activity, but may increase the activity of the enzyme. The amino acid composition is presented and the N-terminal sequence of the 42-kDa polypeptide was found to be: Met-Thr-Pro-Glu-His-Leu-Pro-Thr-Glu-Gln-Tyr-Glu-Ala-Gln-Leu-Ala-Glu-Lys- . The tRNA (m5U54)-methyltransferase has a pI of 4.7 and a pH optimum of 8.0. The enzyme does not require added cations but is stimulated by Mg2+. The apparent Km for tRNA and S-adenosyl-L-methionine are 80 nM and 17 microM, respectively.
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- European Journal of Biochemistry (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Ny, Tor
- Lindström, H R
- Hagervall, T G
- Björk, G R
- Articles in the publication
- European Journal ...
- By the university
- Umeå University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
