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Successful ovulatio...
Successful ovulation in plasminogen-deficient mice treated with the broad-spectrum matrix metalloproteinase inhibitor galardin.
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- Liu, Kui (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Wahlberg, Patrik (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Leonardsson, Göran (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Hägglund, Anna-Carin (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Ny, Annelii (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Bodén, Ida (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Wibom, Carin (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Lund, Leif R (author)
- Finsen Laboratory, Rigshospitalet, Strandboulevarden 49, DK-2100 Copenhagen, Denmark
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- Ny, Tor (author)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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(creator_code:org_t)
- New York : Academic P., 2006
- 2006
- English.
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In: Developmental Biology. - New York : Academic P.. - 0012-1606 .- 1095-564X. ; 295:2, s. 615-622
- Related links:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- Many studies have suggested the hypothesis that the plasminogen activator (PA) system and the matrix metalloproteinase (MMP) system, either separately or in combination, may provide the proteolytic activity that is required for rupture of the follicular wall at the time of ovulation. Our recent studies on ovulation in plasminogen (plg)-deficient mice have, however, shown that plasmin is not required for normal ovulation, leading us to the hypothesis that MMPs may be a more important source of proteolysis for this process. To investigate the role of MMPs and also the possibility of a functional overlap or synergy between the MMP and PA systems during ovulation, we have studied ovulation efficiency in wild-type and plg-deficient mice treated with the broad-spectrum MMP inhibitor galardin. We found that in both wild-type mice and heterozygous plg-deficient (plg(+/-)) mice that had been treated with galardin prior to ovulation, there was a mild (18-20%) reduction in ovulation efficiency. Surprisingly, galardin treatment of plg-deficient (plg(-/-)) mice only caused an additional 14% reduction in ovulation efficiency as compared to vehicle-treated plg(-/-) mice. Our data therefore suggest that although MMPs may play a role in degradation of the follicular wall, they may not be obligatory for ovulation. In contrast to previous studies on tissue remodeling during wound heating and placental development, we have demonstrated that there is no obvious functional overlap or synergy between the PA and MMP systems, which has previously been thought to be essential for the ovulatory process.
Subject headings
- NATURVETENSKAP -- Biologi -- Utvecklingsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Developmental Biology (hsv//eng)
Keyword
- Animals
- Dipeptides/*pharmacology/therapeutic use
- Enzyme Inhibitors/pharmacology/therapeutic use
- Female
- Matrix Metalloproteinases/antagonists & inhibitors/*physiology
- Mice
- Mice; Knockout
- Ovarian Follicle/enzymology/metabolism/ultrastructure
- Ovulation/*drug effects
- Plasminogen/*deficiency
- Plasminogen Activators/physiology
Publication and Content Type
- ref (subject category)
- art (subject category)
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