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Monitoring minimal residual disease with flow cytometry, antigen-receptor gene rearrangements and fusion transcript quantification in Philadelphia-positive childhood acute lymphoblastic leukemia
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- Thörn, Ingrid, 1957- (author)
- Uppsala universitet,Hematologi och immunologi,Molecular Hematology
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- Botling, Johan (author)
- Uppsala universitet,Hematologi och immunologi,Molecular Hematology
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- Hermansson, Monica (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- (creator_code:org_t)
- Elsevier BV, 2009
- 2009
- English.
- In: Leukemia Research. - : Elsevier BV. - 0145-2126 .- 1873-5835. ; 33:8, s. 1047-1054
- Journal article (peer-reviewed)
Abstract
Subject headings
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- In this study, we followed minimal residual disease (MRD) in eight children with Philadelphia-positive acute lymphoblastic leukemia (Ph+ ALL) using (i) flow cytometry (FCM), (ii) real-time quantitative PCR of IG/TCR gene rearrangements and (iii) RT-PCR detecting fusion gene transcripts. In six of the eight cases the kinetics of MRD clearance was comparable. One of the two discordant cases could be explained by presence of an alternative fusion transcript. The other discordant case showed high BCR-ABL1 RNA level while the other methods did not detect any MRD. In our limited material quantitative RT-PCR of fusion gene transcripts seemed particularly useful to measure MRD in Ph+ ALL. However, BCR-ABL1 expression may not reflect the percentage of leukemic cells as FCM and IG/TCR rearrangement quantification do, and these methods are thus complementary.
Keyword
- MRD
- Philadelphia positive ALL
- real-time PCR
- flow cytometry
- antigen receptor genes
- fusion gene transcripts
- BCR-ABL1 mutations
- MEDICINE
- MEDICIN
- Molekylär medicin
- Molecular Medicine
Publication and Content Type
- ref (subject category)
- art (subject category)
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