id:"swepub:oai:DiVA.org:uu-106237"
Search: id:"swepub:oai:DiVA.org:uu-106237" > Expression and puri...
- 1 of 1
- Previous record
- Next record
- To hitlist
Expression and purification of recombinant poly(A)-specific ribonuclease (PARN)
-
- Nilsson, Per (author)
- Uppsala universitet,Molekylärbiologi,Institutionen för cell- och molekylärbiologi
-
- Virtanen, Anders (author)
- Uppsala universitet,Molekylärbiologi
- (creator_code:org_t)
- Elsevier BV, 2006
- 2006
- English.
- In: International Journal of Biological Macromolecules. - : Elsevier BV. - 0141-8130 .- 1879-0003. ; 39, s. 95-99
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- PARN is a poly(A)-specific ribonuclease that degrades the poly(A) tail of mRNA. We have established conditions for expressing soluble recombinant human PARN. We investigated different Escherichia coli strains, expression vectors, media and growth conditions. We found that PARN expressed from pET33 in BL21(DE3) grown in TB and induced at OD595 approximately 1 with 1 mM IPTG yielded mg amounts of soluble PARN per litre culture. Further, a purification protocol was established to purify PARN. We use His-tag affinity chromatography, HiTrap Q HP ion exchange chromatography and 7-Me-GTP-Sepharose affinity chromatography. This purification procedure render a 90-95% pure PARN. Purified recombinant PARN has enzymatic activity and will be used for further mechanistic and structural studies.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Expression of recombinant protein
- His-tag purification
- Ion exchange chromatography
- Affinity purification by 7-Me-GTP-Sepharose
- Poly(A)-specific
- ribonuclease
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- International Journal of Biological Macromolecules (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Nilsson, Per
- Virtanen, Anders
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- Articles in the publication
- International Jo ...
- Int. J. Biol. Ma ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
