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Assisted assembly : how to improve a de novo genome assembly by using related species
- Gnerre, Sante (author)
- Lander, Eric S (author)
-
- Lindblad-Toh, Kerstin (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Jaffe, David B (author)
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- (creator_code:org_t)
- Springer Science and Business Media LLC, 2009
- 2009
- English.
- In: Genome Biology. - : Springer Science and Business Media LLC. - 1465-6906 .- 1474-760X. ; 10:8, s. R88-
- Journal article (peer-reviewed)
Abstract
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- We describe a new assembly algorithm, where a genome assembly with low sequence coverage, either throughout the genome or locally, due to cloning bias, is considerably improved through an assisting process via a related genome. We show that the information provided by aligning the whole-genome shotgun reads of the target against a reference genome can be used to substantially improve the quality of the resulting assembly.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Biology
- Biologi
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- art (subject category)
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