id:"swepub:oai:DiVA.org:uu-119911"
Sökning: id:"swepub:oai:DiVA.org:uu-119911" > Patterns of mRNA ex...
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
Patterns of mRNA expression for matrix molecules and growth factors in flexor tendon injury : differences in the regulation between tendon and tendon sheath
-
- Berglund, Maria, 1975- (författare)
- Uppsala universitet,Handkirurgi
-
- Reno, Carol (författare)
- McCaig Centre for Joint Injury and Artrhitis Research, University of Calgary, Calgary, Canada
-
- Hart, David A. (författare)
- McCaig Centre for Joint Injury and Arthritis Research, University of Calgary, Calgary, Canada
- visa fler...
- visa färre...
- (creator_code:org_t)
- Elsevier BV, 2006
- 2006
- Engelska.
- Ingår i: Journal of Hand Surgery-American Volume. - : Elsevier BV. - 0363-5023 .- 1531-6564. ; 31A:8, s. 1279-1287
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- PURPOSE: Injuries to tendons, particularly flexor tendons, can lead to loss of function after healing due to adhesion formation and other complications. The aim of this study was to increase our understanding of the healing process in tendons and tendon sheaths to develop methods to affect the healing process and improve the outcome of tendon repair in the future. METHODS: In a rabbit model of flexor tendon injury, tissues were harvested 3, 6, 12, and 24 days after surgery (n = 6 for each group). After RNA extraction, messenger RNA (mRNA) levels for relevant genes in tendon and tendon sheaths were measured using the reverse transcription polymerase chain reaction. Messenger RNA levels for a subset of relevant molecules at different time points after injury were compared with those of uninjured controls for tendons and tendon sheaths. RESULTS: Initially after injury, there was a shift in collagen expression with a marked increase in type III mRNA levels in both the tendon and tendon sheath, whereas those for collagen I increased only in the sheath at later time points. Aggrecan and versican mRNA levels were increased in both tissues, but temporal aspects of the changes were different. The mRNA levels for biglycan and lumican were all upregulated throughout the healing interval examined, whereas those for decorin were significantly decreased throughout in the tendon more so than the sheath. The mRNA levels for basic fibroblastic growth factor and transforming growth factor beta were elevated after injury in the tendon but not in the sheath. In contrast, mRNA levels for connective tissue growth factor were unaltered or decreased in both tissues throughout the interval assessed. CONCLUSIONS: Healing after injury to the rabbit flexor tendon and tendon sheath follow a reproducible pattern of gene expression; however, the pattern in the tendon is very different from that in the sheath. These findings indicate that interventions developed to improve healing of these tissues will have to address these differences, because they will likely affect the outcomes.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Nyckelord
- bFGF
- collagen
- flexor tendon
- proteoglycan
- TGF-B1
- Hand surgery
- Handkirurgi
- Orthopaedics
- Ortopedi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
- Journal of Hand Surgery-American Volume (Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- 1 av 1
- Föregående post
- Nästa post
- Till träfflistan
Hitta mer i SwePub
- Av författaren/redakt...
- Berglund, Maria, ...
- Reno, Carol
- Hart, David A.
- Wiig, Monica
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
- MEDICIN OCH HÄLS ...
- och Klinisk medicin
- och Kirurgi
- Artiklar i publikationen
- Journal of Hand ...
- Av lärosätet
- Uppsala universitet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
