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Sökning: id:"swepub:oai:DiVA.org:uu-132137" > H2O2-induced Ca2+ i...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003397naa a2200493 4500
001oai:DiVA.org:uu-132137
003SwePub
008101015s2009 | |||||||||||000 ||eng|
009oai:prod.swepub.kib.ki.se:119993110
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1321372 URI
0247 a https://doi.org/10.1111/j.1582-4934.2009.00737.x2 DOI
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:1199931102 URI
040 a (SwePub)uud (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Bari, Muhammad R.4 aut
2451 0a H2O2-induced Ca2+ influx and its inhibition by N-(p-amylcinnamoyl) anthranilic acid in the beta-cells :b involvement of TRPM2 channels
264 c 2009-03-06
264 1b Wiley,c 2009
338 a print2 rdacarrier
520 a Type 2 melastatin-related transient receptor potential channel (TRPM2), a member of the melastatin-related TRP (transient receptor potential) subfamily is a Ca(2+)-permeable channel activated by hydrogen peroxide (H(2)O(2)). We have investigated the role of TRPM2 channels in mediating the H(2)O(2)-induced increase in the cytoplasmic free Ca(2+) concentration ([Ca(2+)](i)) in insulin-secreting cells. In fura-2 loaded INS-1E cells, a widely used model of beta-cells, and in human beta-cells, H(2)O(2) increased [Ca(2+)](i), in the presence of 3 mM glucose, by inducing Ca(2+) influx across the plasma membrane. H(2)O(2)-induced Ca(2+) influx was not blocked by nimodipine, a blocker of the L-type voltage-gated Ca(2+) channels nor by 2-aminoethoxydiphenyl borate, a blocker of several TRP channels and store-operated channels, but it was completely blocked by N-(p-amylcinnamoyl)anthranilic acid (ACA), a potent inhibitor of TRPM2. Adenosine diphosphate phosphate ribose, a specific activator of TRPM2 channel and H(2)O(2), induced inward cation currents that were blocked by ACA. Western blot using antibodies directed to the epitopes on the N-terminal and on the C-terminal parts of TRPM2 identified the full length TRPM2 (TRPM2-L), and the C-terminally truncated TRPM2 (TRPM2-S) in human islets. We conclude that functional TRPM2 channels mediate H(2)O(2)-induced Ca(2+) entry in beta-cells, a process potently inhibited by ACA.
653 a Calcium influx
653 a Calcium signalling
653 a Insulin-secreting cells
653 a Microfluorometry
653 a N-(p-amylcinnamoyl)anthranilic acid
653 a TRP channels
653 a TRPM2
653 a MEDICINE
653 a MEDICIN
7001 a Akbar, Sanian4 aut
7001 a Eweida, Mohamed4 aut
7001 a Kühn, Frank J. P.4 aut
7001 a Gustafsson, Amanda Jabin4 aut
7001 a Lückhoff, Andreas4 aut
7001 a Islam, M. Shahidulu Karolinska Institutet,Uppsala universitet,Medicin4 aut
7102 a Uppsala universitetb Medicin4 org
7730 t Journal of Cellular and Molecular Medicine (Print)d : Wileyg 13:9B, s. 3260-3267q 13:9B<3260-3267x 1582-1838x 1582-4934
8564 u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1582-4934.2009.00737.x
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-132137
8564 8u https://doi.org/10.1111/j.1582-4934.2009.00737.x
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:119993110

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