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Proteolytic modific...
Proteolytic modification of pig and rat liver pyruvate kinase including the phosphorylatable site
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- Bergström, Gunnel (author)
- Uppsala universitet,Institutionen för medicinsk och fysiologisk kemi,Engström Lorentz
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- Ekman, Pia (author)
- Uppsala universitet,Institutionen för medicinsk och fysiologisk kemi,Engström Lorentz
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- Humble, Elisabet (author)
- Uppsala universitet,Institutionen för medicinsk och fysiologisk kemi,Engström Lorentz
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show more...
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- Engström, Lorentz (author)
- Uppsala universitet,Institutionen för medicinsk och fysiologisk kemi,Engström Lorentz
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show less...
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(creator_code:org_t)
- 1978
- 1978
- English.
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In: Biochimica et Biophysica Acta. - 0006-3002 .- 1878-2434. ; 532:2, s. 259-267
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- The phosphorylated or phosphate-accepting site of pyruvate kinase from pig and rat liver was removed without inactivation by incubation with subtilisin. At different time intervals the subtilisin was inactivated with phenylmethylsulfonyl fluoride and the amount of remaining phosphorylatable or phosphorylated sites of pyruvate kinase estimated by incubation with an excess of [32P]-ATP and protein kinase. It was found that to get the same rate of modification the subtilisin concentration required to modify unphosphorylated pyruvate kinase was approximately ten times higher than that used for removal of the phosphorylated site of phosphorylated site of phosphorylated enzyme. It was shown that the proteolytically-modified pyruvate kinase had an increased apparent Km for phosphoenolpyruvate without a change in V, when compared to unmodified unphosphorylated and phosphorylated pyruvate kinase. The removal of the phosphorylated site was not associated with loss of the allosteric sites for ATP and Fru-1,6-P2. The possibility that phosphorylation of the pyruvate kinase increases its degradation rate in vivo is briefly discussed.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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