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Acoustically enriching, large-depth aquatic sampler

Jonsson, Jonas (author)
Uppsala universitet,Mikrosystemteknik,ÅSTC
Ogden, Sam (author)
Uppsala universitet,Mikrosystemteknik
Johansson, Linda (author)
Uppsala universitet,Mikrosystemteknik
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Hjort, Klas (author)
Uppsala universitet,Mikrosystemteknik
Thornell, Greger (author)
Uppsala universitet,Mikrosystemteknik,ÅSTC
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 (creator_code:org_t)
2012
2012
English.
In: Lab on a Chip. - 1473-0197 .- 1473-0189. ; 12:9, s. 1619-1628
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • In marine biology, it is useful to collect water samples when exploring the distribution and diversity of microbial communities in underwater environments. In order to provide, e.g., a miniaturized submersible explorer with the capability of collecting microorganisms, a compact sample enrichment system has been developed. The sampler is 30 mm long, 15 mm wide, and just a few millimetres thick. Integrated in a multilayer steel, polyimide and glass construction is a microfluidic channel with piezoelectric transducers, where microorganism and particle samples are collected and enriched, using acoustic radiation forces for gentle and labelless trapping. High-pressure, latchable valves, using paraffin as the actuation material, at each end of the microfluidic channel keep the collected sample pristine. A funnel structure raised above the surface of the device directs water into the microfluidic channel as the vehicle propels itself or when there is a flow across its hull. The valves proved leak proof to a pressure of 2.1 MPa for 19 hours and momentary pressures of 12.5 MPa, corresponding to an ocean depth of more than 1200 metres. By reactivating the latching mechanism, small leakages through the valves could be remedied, which could thus increase the leak-less operational time. Fluorescent particles, 1.9 µm in diameter, were successfully trapped in the microfluidic channel at flow rates up to 15 ml min-1, corresponding to an 18.5 cm s-1 external flow rate of the sampler. In addition, liquid-suspended GFP-marked yeast cells were successfully trapped.

Keyword

Acoustic
microorganism
enriching
trap
valve
paraffin
sampler
marine
actuator
TECHNOLOGY
TEKNIKVETENSKAP
Teknisk fysik med inriktning mot mikrosystemteknik
Engineering Science with specialization in Microsystems Technology

Publication and Content Type

ref (subject category)
art (subject category)

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