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In-vivo extravasati...
In-vivo extravasation induces the expression of interleukin 1 receptor type 1 in human neutrophils
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- Paulsson, J. M. (author)
- Karolinska Institutet
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- Moshfegh, A. (author)
- Karolinska Institutet
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Dadfar, E. (author)
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- Held, Claes (author)
- Uppsala universitet,Uppsala kliniska forskningscentrum (UCR)
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- Jacobson, S. H. (author)
- Karolinska Institutet
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- Lundahl, J. (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2012-03-02
- 2012
- English.
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In: Clinical and Experimental Immunology. - : Oxford University Press (OUP). - 0009-9104 .- 1365-2249. ; 168:1, s. 105-112
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- In order to address neutrophil activation during inflammation we assessed the expression of interleukin 1 receptor type 1 (IL-1R1) following in-vivo extravasation. Extravasated neutrophils were collected from 11 healthy study subjects by a skin chamber technique and compared to neutrophils in peripheral blood. Expression of IL-1R1 was assessed by microarray, quantitative polymerase chain reaction (qPCR), Western blot, flow cytometry, enzyme linked immunosorbent assay (ELISA) and immunoelectron microscopy (iEM). IL-1R1 was induced following extravasation, demonstrated by both gene array and qPCR. Western blot demonstrated an increased expression of IL-1R1 in extravasated leucocytes. This was confirmed further in neutrophils by flow cytometry and iEM that also demonstrated an increased intracellular pool of IL-1R1 that could be mobilized by N-formyl-methionine-leucine-phenylalanine (fMLP). Stimulation of peripheral neutrophils with IL-1 resulted in transcription of NF?B and a number of downstream chemokines and the corresponding chemokines were also induced following in-vivo extravasation. The present results demonstrate that IL-1R1 is induced following extravasation and exists on the neutrophil surface, as well as in a mobile intracellular pool. Furthermore, neutrophils express functional IL-1R1 as demonstrated by the induction of chemokines following IL-1 stimulation. The results indicate a potential role for IL-1 in the activation of neutrophils at inflammatory sites.
Keyword
- extravasation
- IL-1R1
- neutrophils
Publication and Content Type
- ref (subject category)
- art (subject category)
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