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Model studies on ir...
Model studies on iron (III) ion affinity chromatography : II. Interaction of immobilized ferric ions with phosphorylated amino acids, peptides and proteins
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- Muszynska, Grazyna (author)
- Polish Academy of Science, Warsawa,Grazyna Muszynska
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- Dobrowolska, Grazyna (author)
- Polish Academy of Science, Warsawa,Grazyna Muszynska
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- Medin, Anders (author)
- Uppsala universitet,Biokemi,Jerker Porath
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- Ekman, Pia (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,ek pia
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- Porath, Jerker (author)
- Uppsala universitet,Biokemi,Jerker Porath
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(creator_code:org_t)
- 1992
- 1992
- English.
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In: Journal of Chromatography A. - 0021-9673 .- 1873-3778. ; 604:1, s. 19-28
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The chromatographic behaviour of phosphoamino acids, phosphopeptides and phosphoproteins and their non-phosphorylated counterparts was studied on Fe(III)-Chelating Sepharose and Fe(III)-Chelating Superose. The phosphorylated compounds, in contrast to their non-phosphorylated or dephosphorylated counterparts, adsorb to immobilized iron(III) ions at pH 5.5 and can be desorbed by an increase in pH. Phosphoamino acids were eluted at pH 6.5-6.7, whereas monophosphopeptides and phosphoprotamine eluted in the pH range 6.9-7.5. Molecules possessing clusters(s) of carboxylic groups are weakly retained (gamma-carboxyglutamic acid, Ala-Ser-Glu5) or bound (polyglutamic acid, beta-casein) to the immobilized iron(III) ions at pH 5.5. Dephosphorylated beta-casein was desorbed at pH 7.0, whereas for elution of native (non-dephosphorylated) beta-casein, phosphate buffer of pH 7.7 was required. The homopolymer of polyglutamic acid was desorbed in the pH range 6.0-6.3, whereas copolymers of glutamic acid and tyrosine require pH 7.0-7.3 or even phosphate buffer at pH 7.7 for elution.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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