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Improving the Cell ...
Improving the Cell Distribution in Collagen-Coated Poly-Caprolactone Knittings
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Sun, Weilun (author)
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Tiemessen, Dorien M. (author)
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Sloff, Marije (author)
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Lammers, Rianne J. (author)
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de Mulder, Eric L. W. (author)
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- Hilborn, Jöns, 1956- (author)
- Uppsala universitet,Polymerkemi
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Gupta, Bhuvanesh (author)
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Feitz, Wout F. J. (author)
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Daamen, Willeke F. (author)
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van Kuppevelt, Toin H. (author)
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Geutjes, Paul J. (author)
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Oosterwijk, Egbert (author)
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(creator_code:org_t)
- Mary Ann Liebert Inc, 2012
- 2012
- English.
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In: TISSUE ENG PART C-ME. - : Mary Ann Liebert Inc. - 1937-3384. ; 18:10, s. 731-739
- Related links:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Adequate cellular in-growth into biomaterials is one of the fundamental requirements of scaffolds used in regenerative medicine. Type I collagen is the most commonly used material for soft tissue engineering, because it is nonimmunogenic and a highly porous network for cellular support can be produced. However, in general, adequate cell in-growth and cell seeding has been suboptimal. In this study we prepared collagen scaffolds of different collagen densities and investigated the cellular distribution. We also prepared a hybrid polymer-collagen scaffold to achieve an optimal cellular distribution as well as sufficient mechanical strength. Collagen scaffolds [ranging from 0.3% to 0.8% (w/v)] with and without a mechanically stable polymer knitting [polycaprolactone (PCL)] were prepared. The porous structure of collagen scaffolds was characterized using scanning electron microscopy and hematoxylin-eosin staining. The mechanical strength of hybrid scaffolds (collagen with or without PCL) was determined using tensile strength analysis. Cellular in-growth and interconnectivity were evaluated using fluorescent bead distribution and human bladder smooth muscle cells and human urothelium seeding. The lower density collagen scaffolds showed remarkably deeper cellular penetration and by combining it with PCL knitting the tensile strength was enhanced. This study indicated that a hybrid scaffold prepared from 0.4% collagen strengthened with knitting achieved the best cellular distribution.
Subject headings
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
Keyword
- Polymer chemistry
- Polymerkemi
- Kemi med inriktning mot polymerkemi
- Chemistry with specialization in Polymer Chemistry
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Sun, Weilun
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Tiemessen, Dorie ...
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Sloff, Marije
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Lammers, Rianne ...
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de Mulder, Eric ...
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Hilborn, Jöns, 1 ...
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show more...
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Gupta, Bhuvanesh
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Feitz, Wout F. J ...
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Daamen, Willeke ...
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van Kuppevelt, T ...
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Geutjes, Paul J.
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Oosterwijk, Egbe ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Polymer Chemistr ...
- Articles in the publication
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TISSUE ENG PART ...
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Tissue Engineeri ...
- By the university
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Uppsala University