Search: id:"swepub:oai:DiVA.org:uu-188407" >
Oxygen-charged HTK-...
Oxygen-charged HTK-F6H8 emulsion reduces ischemia : reperfusion injury in kidneys from brain-dead pigs
-
- Asif, Sana (author)
- Uppsala universitet,Klinisk immunologi,korsgren
-
- Sedigh, Amir (author)
- Uppsala universitet,Transplantationskirurgi
-
- Nordström, Johan (author)
- Karolinska Institutet
-
show more...
-
- Brandhorst, Heide (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
-
- Jorns, Carl (author)
- Karolinska Institutet
-
- Lorant, Tomas (author)
- Uppsala universitet,Transplantationskirurgi
-
- Larsson, Erik (author)
- Uppsala universitet,Molekylär och morfologisk patologi,alafuzoff
-
- Magnusson, Peetra U. (author)
- Uppsala universitet,Klinisk immunologi,magnusson
-
- Nowak, Greg (author)
- Karolinska Institutet
-
- Theisinger, Sonja (author)
- Novaliq GmbH, Heidelberg, Germany
-
- Hoeger, Simone (author)
- Department of Nephrology, Endocrinology and Rheumatology, University Medical Center Mannheim, Mannheim, Germany
-
- Wennberg, Lars (author)
- Karolinska Institutet
-
- Korsgren, Olle (author)
- Uppsala universitet,Klinisk immunologi,korsgren
-
- Brandhorst, Daniel (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2012
- 2012
- English.
-
In: Journal of Surgical Research. - : Elsevier BV. - 0022-4804 .- 1095-8673. ; 178:2, s. 959-967
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
http://kipublication...
-
show less...
Abstract
Subject headings
Close
- Background:Prolonged cold ischemia is frequently associated with a greater risk of delayed graft function and enhanced graft failure. We hypothesized that media, combining a high oxygen-dissolving capacity with specific qualities of organ preservation solutions, would be more efficient in reducing immediate ischemia-reperfusion injury from organs stored long term compared with standard preservation media.Methods:Kidneys retrieved from brain-dead pigs were flushed using either cold histidine-tryptophan-ketoglutarate (HTK) or oxygen-precharged emulsion composed of 75% HTK and 25% perfluorohexyloctane. After 18 h of cold ischemia the kidneys were transplanted into allogeneic recipients and assessed for adenosine triphosphate content, morphology, and expression of genes related to hypoxia, environmental stress, inflammation, and apoptosis.Results:Compared with HTK-flushed kidneys, organs preserved using oxygen-precharged HTK-perfluorohexyloctane emulsion had increased elevated adenosine triphosphate content and a significantly lower gene expression of hypoxia inducible factor-1 alpha, vascular endothelial growth factor, interleukin-1 alpha, tumor necrosis factor-alpha, interferon-alpha, JNK-1, p38, cytochrome-c, Bax, caspase-8, and caspase-3 at all time points assessed. In contrast, the mRNA expression of Bcl-2 was significantly increased.Conclusions:The present study has demonstrated that in brain-dead pigs the perfusion of kidneys with oxygen-precharged HTK-perfluorohexyloctane emulsion results in significantly reduced inflammation, hypoxic injury, and apoptosis and cellular integrity and energy content are well maintained. Histologic examination revealed less tubular, vascular, and glomerular changes in the emulsion-perfused tissue compared with the HTK-perfused counterparts. The concept of perfusing organs with oxygen-precharged emulsion based on organ preservation media represents an efficient alternative for improved organ preservation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Keyword
- Brain death
- Emulsion
- Kidney transplantation
- Organ preservation
- Oxygen carrier
- Pig
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Asif, Sana
-
Sedigh, Amir
-
Nordström, Johan
-
Brandhorst, Heid ...
-
Jorns, Carl
-
Lorant, Tomas
-
show more...
-
Larsson, Erik
-
Magnusson, Peetr ...
-
Nowak, Greg
-
Theisinger, Sonj ...
-
Hoeger, Simone
-
Wennberg, Lars
-
Korsgren, Olle
-
Brandhorst, Dani ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Surgery
- Articles in the publication
-
Journal of Surgi ...
- By the university
-
Uppsala University
-
Karolinska Institutet