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Molecular characterization of neoplastic and normal "sister" lymphoblastoid B-cell lines from chronic lymphocytic leukemia

Myhrinder, Anna Lanemo, 1975- (author)
Linköpings universitet,Hälsouniversitetet,Cellbiologi
Hellqvist, Eva (author)
Linköpings universitet,Hälsouniversitetet,Cellbiologi
Bergh, Ann-Charlotte (author)
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Jansson, Mattias (author)
Uppsala universitet,Hematologi och immunologi,Department of Genetics and Pathology, Uppsala University, SE-781 85 Uppsala, Sweden
Nilsson, Kenneth (author)
Uppsala universitet,Hematologi och immunologi,Department of Genetics and Pathology, Uppsala University, SE-781 85 Uppsala, Sweden
Hultman, Per (author)
Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Molekylär och immunologisk patologi,Klinisk patologi och klinisk genetik
Jonasson, Jon (author)
Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Molekylär och immunologisk patologi,Klinisk patologi och klinisk genetik
Buhl, Anne Mette (author)
Pedersen, Lone Bredo (author)
Jurlander, Jesper (author)
Klein, Eva (author)
Karolinska Institutet
Weit, Nicole (author)
Herling, Marco (author)
Rosenquist, Richard (author)
Uppsala universitet,Hematologi och immunologi,Department of Genetics and Pathology, Uppsala University, SE-781 85 Uppsala, Sweden
Rosén, Anders (author)
Linköpings universitet,Hälsouniversitetet,Cellbiologi
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 (creator_code:org_t)
2013-02-25
2013
English.
In: Leukemia and Lymphoma. - : Informa UK Limited. - 1042-8194 .- 1029-2403. ; 54:8, s. 1769-1779
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Chronic lymphocytic leukemia (CLL) B-cells resemble self-renewing CD5 + B-cells carrying auto/xeno-antigen-reactive B-cell receptors (BCRs) and multiple innate pattern-recognition receptors, such as Toll-like receptors and scavenger receptors. Integration of signals from BCRs with multiple surface membrane receptors determines whether the cells will be proliferating, anergic or apoptotic. To better understand the role of antigen in leukemogenesis, CLL cell lines producing monoclonal antibodies (mAbs) will facilitate structural analysis of antigens and supply DNA for genetic studies. We present here a comprehensive genotypic and phenotypic characterization of available CLL and normal B-cell-derived lymphoblastoid cell lines (LCLs) from the same individuals (n = 17). Authenticity and verification studies of CLL-patient origin were done by IGHV sequencing, fluorescence in situ hybridization (FISH) and DNA/short tandem repeat (STR) fingerprinting. Innate B-cell features, i.e. natural Ab production and CD5 receptors, were present in most CLL cell lines, but in none of the normal LCLs. This panel of immortalized CLL-derived cell lines is a valuable reference representing a renewable source of authentic Abs and DNA.

Keyword

Chronic lymphocytic leukemia (CLL)
lymphoblastoid cell line (LCL)
IGHV sequence
cell-of-origin
natural antibodies
antigen structure
CD5+innate B-cells
MEDICINE

Publication and Content Type

ref (subject category)
art (subject category)

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