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Mass spectrometry i...
Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection
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- Hulme, Heather E. (author)
- Univ Glasgow, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow G12 8QQ, Lanark, Scotland.
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- Meikle, Lynsey M. (author)
- Univ Glasgow, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow G12 8QQ, Lanark, Scotland.
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- Wessel, Hannah (author)
- Univ Glasgow, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow G12 8QQ, Lanark, Scotland.
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- Strittmatter, Nicole (author)
- AstraZeneca, Milton Sci Pk, Cambridge CB4 0WG, England.
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- Swales, John (author)
- AstraZeneca, Milton Sci Pk, Cambridge CB4 0WG, England.
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- Thomson, Carolyn (author)
- Univ Glasgow, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow G12 8QQ, Lanark, Scotland.
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- Nilsson, Anna (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Nibbs, Robert J. B. (author)
- Univ Glasgow, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow G12 8QQ, Lanark, Scotland.
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Milling, Simon (author)
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- Andrén, Per E. (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Mackay, C. Logan (author)
- Univ Edinburgh, Sch Chem, Edinburgh EH9 3FJ, Midlothian, Scotland.
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- Dexter, Alex (author)
- Natl Phys Lab, Teddington TW11 0LW, Middx, England.
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- Bunch, Josephine (author)
- Natl Phys Lab, Teddington TW11 0LW, Middx, England.
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- Goodwin, Richard J. A. (author)
- AstraZeneca, Milton Sci Pk, Cambridge CB4 0WG, England.
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- Burchmore, Richard (author)
- Univ Glasgow, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow G12 8QQ, Lanark, Scotland.
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- Wall, Daniel M. (author)
- Univ Glasgow, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow G12 8QQ, Lanark, Scotland.
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Univ Glasgow, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow G12 8QQ, Lanark, Scotland AstraZeneca, Milton Sci Pk, Cambridge CB4 0WG, England. (creator_code:org_t)
- 2017-06-05
- 2017
- English.
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In: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 7
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Abstract
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- Salmonella Typhimurium causes a self-limiting gastroenteritis that may lead to systemic disease. Bacteria invade the small intestine, crossing the intestinal epithelium from where they are transported to the mesenteric lymph nodes (MLNs) within migrating immune cells. MLNs are an important site at which the innate and adaptive immune responses converge but their architecture and function is severely disrupted during S. Typhimurium infection. To further understand host-pathogen interactions at this site, we used mass spectrometry imaging (MSI) to analyse MLN tissue from a murine model of S. Typhimurium infection. A molecule, identified as palmitoylcarnitine (PalC), was of particular interest due to its high abundance at loci of S. Typhimurium infection and MLN disruption. High levels of PalC localised to sites within the MLNs where B and T cells were absent and where the perimeter of CD169(+) sub capsular sinus macrophages was disrupted. MLN cells cultured ex vivo and treated with PalC had reduced CD4(+) CD25(+) T cells and an increased number of B220(+) CD19(+) B cells. The reduction in CD4(+) CD25(+) T cells was likely due to apoptosis driven by increased caspase-3/7 activity. These data indicate that PalC significantly alters the host response in the MLNs, acting as a decisive factor in infection outcome.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Läkemedelsbioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Pharmaceutical Biotechnology (hsv//eng)
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Hulme, Heather E ...
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Meikle, Lynsey M ...
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Wessel, Hannah
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Strittmatter, Ni ...
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Swales, John
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Thomson, Carolyn
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Nilsson, Anna
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Nibbs, Robert J. ...
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Milling, Simon
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Andrén, Per E.
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Mackay, C. Logan
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Dexter, Alex
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Bunch, Josephine
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Goodwin, Richard ...
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Burchmore, Richa ...
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Wall, Daniel M.
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