id:"swepub:oai:DiVA.org:uu-369398"
Sökning: id:"swepub:oai:DiVA.org:uu-369398" > Isotopic resonance ...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03338naa a2200397 4500 | |
| 001 | oai:DiVA.org:uu-369398 | |
| 003 | SwePub | |
| 008 | 181212s2018 | |||||||||||000 ||eng| | |
| 009 | oai:prod.swepub.kib.ki.se:139492571 | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3693982 URI |
| 024 | 7 | a https://doi.org/10.1038/s41598-018-34704-02 DOI |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1394925712 URI |
| 040 | a (SwePub)uud (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Rodin, Sergeyu Karolinska Institutet,Uppsala universitet,Institutionen för kirurgiska vetenskaper,Karolinska Inst, Dept Med Biochem & Biophys, Div Physiol Chem 1, Scheelesvag 2, SE-17177 Stockholm, Sweden4 aut0 (Swepub:uu)serro142 |
| 245 | 1 0 | a Isotopic resonance at 370 ppm deuterium negatively affects kinetics of luciferin oxidation by luciferase |
| 264 | c 2018-11-02 | |
| 264 | 1 | b NATURE PUBLISHING GROUP,c 2018 |
| 338 | a electronic2 rdacarrier | |
| 520 | a Since 1930s, it has been known that some biochemical and biological processes exhibit abnormal kinetics at a deuterium concentration in the local environment of 250-600 ppm, which is 2-4 times higher that the normal concentration of 150 ppm D. We sought to test if the kinetics of firefly luciferase oxidizing luciferin, the reaction widely used as a read-out in various biochemical assays, is also affected by an elevated deuterium content. To this end, both luciferase and luciferin substrate solutions were prepared based on water with extra deuterium added to a concentration ranging from 150 ppm and up to 10,000 ppm (1%). Upon mixing the solutions, the luminescence intensity at different times was compared with that of the corresponding control solutions with 150 ppm D. A broad negative resonance was detected (p < 10(-6)), with a approximate to 20% drop in luminescence at 370 ppm D. Given that, on average, about half of hydrogen atoms in proteins are not exchangeable in solution, this value corresponds to approximate to 260 ppm of deuterium in all enzyme's hydrogens, in a very good agreement with the prediction of the Isotopic resonance hypothesis. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 700 | 1 | a Rebellato, Paolau Biothema AB, Handens Stn Vag 17, S-13640 Handen, Sweden4 aut |
| 700 | 1 | a Lundin, Arneu Biothema AB, Handens Stn Vag 17, S-13640 Handen, Sweden4 aut |
| 700 | 1 | a Zubarev, Roman A.u Karolinska Institutet4 aut |
| 710 | 2 | a Uppsala universitetb Institutionen för kirurgiska vetenskaper4 org |
| 773 | 0 | t Scientific Reportsd : NATURE PUBLISHING GROUPg 8q 8x 2045-2322 |
| 856 | 4 | u https://doi.org/10.1038/s41598-018-34704-0y Fulltext |
| 856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1270298/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
| 856 | 4 | u https://www.nature.com/articles/s41598-018-34704-0.pdf |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-369398 |
| 856 | 4 8 | u https://doi.org/10.1038/s41598-018-34704-0 |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:139492571 |
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