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Divergent Effects o...
Divergent Effects of Acute and Prolonged Interleukin 33 Exposure on Mast Cell IgE-Mediated Functions
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- Rönnberg, Elin (författare)
- Karolinska Institutet,Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Immunol & Allergy Unit, Solna, Sweden
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- Ghaib, Avan (författare)
- Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Immunol & Allergy Unit, Solna, Sweden;Univ Sulaimani, Coll Med, Dept Microbiol, Sulaimani, Iraq
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- Ceriol, Carlos (författare)
- Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Immunol & Allergy Unit, Solna, Sweden
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- Enoksson, Mattias (författare)
- Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Immunol & Allergy Unit, Solna, Sweden
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- Arock, Michel (författare)
- CNRS, UMR 8113, Ecole Normale Super Cachan, Mol & Cellular Oncol,LBPA, Cachan, France;Grp Hosp Pitie Salpetriere, Lab Cent Hematol, Paris, France
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- Säfholm, Jesper (författare)
- Karolinska Institutet,Karolinska Inst, Inst Environm Med, Unit Asthma & Allergy Res, Solna, Sweden
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Orre, Ann-Charlotte (författare)
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- Al-Ameri, Mamdoh (författare)
- Karolinska Institutet
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- Adner, Mikael (författare)
- Karolinska Institutet
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- Dahlen, Sven-Erik (författare)
- Karolinska Institutet
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- Ekoff, Maria (författare)
- Karolinska Institutet,Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Immunol & Allergy Unit, Solna, Sweden
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- Nilsson, Gunnar (författare)
- Uppsala universitet,Hematologi,Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Immunol & Allergy Unit, Solna, Sweden
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(creator_code:org_t)
- 2019-06-19
- 2019
- Engelska.
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Ingår i: Frontiers in Immunology. - : FRONTIERS MEDIA SA. - 1664-3224. ; 10
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://www.frontier...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Background: Epithelial cytokines, including IL-33 and Thymic stromal lymphopoietin (TSLP), have attracted interest because of their roles in chronic allergic inflammation-related conditions such as asthma. Mast cells are one of the major targets of IL-33, to which they respond by secreting cytokines. Most studies performed thus far have investigated the acute effects of IL-33 on mast cells. In the current study, we investigated how acute vs. prolonged exposure of mast cells to IL-33 and TSLP affects mediator synthesis and IgE-mediated activation.Methods: Human lung mast cells (HLMCs), cord blood-derived mast cells (CBMCs), and the ROSA mast cell line were used for this study. Receptor expression and the levels of mediators were measured after treatment with IL-33 and/or TSLP.Results: IL-33 induced the release of cytokines. Prolonged exposure to IL-33 increased while TSLP reduced intracellular levels of tryptase. Acute IL-33 treatment strongly potentiated IgE-mediated activation. In contrast, 4 days of exposure to IL-33 decreased IgE-mediated activation, an effect that was accompanied by a reduction in Fc epsilon RI expression.Conclusion: We show that IL-33 plays dual roles in mast cells, in which its acute effects include cytokine release and the potentiation of IgE-mediated degranulation, whereas prolonged exposure to IL-33 reduces IgE-mediated activation. We conclude that mast cells act quickly in response to the alarmin IL-33 to initiate an acute inflammatory response, whereas extended exposure to IL-33 during prolonged inflammation reduces IgE-mediated responses. This negative feedback effect suggests the presence of a novel regulatory pathway that modulates IgE-mediated human mast cell responses.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Fc epsilon RI
- IgE
- IL-33
- mast cells
- TSLP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Rönnberg, Elin
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Ghaib, Avan
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Ceriol, Carlos
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Enoksson, Mattia ...
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Arock, Michel
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Säfholm, Jesper
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visa fler...
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Orre, Ann-Charlo ...
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Al-Ameri, Mamdoh
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Adner, Mikael
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Dahlen, Sven-Eri ...
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Ekoff, Maria
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Nilsson, Gunnar
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visa färre...
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Karolinska Institutet