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Impact of Q-Griffit...
Impact of Q-Griffithsin anti-HIV microbicide gel in non-human primates : In situ analyses of epithelial and immune cell markers in rectal mucosa
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- Günaydin, Gökce (författare)
- Karolinska Institutet
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- Edfeldt, Gabriella (författare)
- Karolinska Univ Hosp, Karolinska Inst, Ctr Mol Med, Dept Med Solna,Div Infect Dis, Stockholm, Sweden
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- Garber, David A. (författare)
- CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA
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- Asghar, Muhammad (författare)
- Karolinska Institutet
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- Noel-Romas, Laura (författare)
- Publ Hlth Agcy Canada, JC Wilt Infect Dis Res Ctr, Natl HIV & Retrovirol Labs, Winnipeg, MB, Canada;Univ Manitoba, Dept Obstet & Gynecol, Winnipeg, MB, Canada;Univ Manitoba, Dept Med Microbiol, Winnipeg, MB, Canada
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- Burgener, Adam (författare)
- Karolinska Institutet
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- Wählby, Carolina, professor, 1974- (författare)
- Uppsala universitet,Avdelningen för visuell information och interaktion,Science for Life Laboratory, SciLifeLab,Bildanalys och människa-datorinteraktion
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- Wang, Lin (författare)
- Magee Womens Res Inst, Pittsburgh, PA USA
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- Rohan, Lisa C. (författare)
- Magee Womens Res Inst, Pittsburgh, PA USA;Univ Pittsburgh, Sch Pharm, Pittsburgh, PA USA
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- Guenthner, Patricia (författare)
- CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA
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- Mitchell, James (författare)
- CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA
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- Matoba, Nobuyuki (författare)
- Univ Louisville, Ctr Predict Med, Louisville, KY 40292 USA;Univ Louisville, Dept Pharmacol & Toxicol, Louisville, KY 40292 USA;Univ Louisville, James Graham Brown Canc Ctr, Louisville, KY 40292 USA
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- McNicholl, Janet M. (författare)
- CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA
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- Palmer, Kenneth E. (författare)
- Univ Louisville, Ctr Predict Med, Louisville, KY 40292 USA;Univ Louisville, Dept Pharmacol & Toxicol, Louisville, KY 40292 USA;Univ Louisville, James Graham Brown Canc Ctr, Louisville, KY 40292 USA
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- Tjernlund, Annelie (författare)
- Karolinska Institutet
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- Broliden, Kristina (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2019-12-02
- 2019
- Engelska.
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Ingår i: Scientific Reports. - : NATURE PUBLISHING GROUP. - 2045-2322. ; 9
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Natural-product derived lectins can function as potent viral inhibitors with minimal toxicity as shown in vitro and in small animal models. We here assessed the effect of rectal application of an anti-HIV lectin-based microbicide Q-Griffithsin (Q-GRFT) in rectal tissue samples from rhesus macaques. E-cadherin(+) cells, CD4(+) cells and total mucosal cells were assessed using in situ staining combined with a novel customized digital image analysis platform. Variations in cell numbers between baseline, placebo and Q-GRFT treated samples were analyzed using random intercept linear mixed effect models. The frequencies of rectal E-cadherin(+) cells remained stable despite multiple tissue samplings and Q-GRFT gel (0.1%, 0.3% and 1%, respectively) treatment. Whereas single dose application of Q-GRFT did not affect the frequencies of rectal CD4(+) cells, multi-dose Q-GRFT caused a small, but significant increase of the frequencies of intra-epithelial CD4(+) cells (placebo: median 4%; 1% Q-GRFT: median 7%) and of the CD4(+) lamina propria cells (placebo: median 30%; 0.1-1% Q-GRFT: median 36-39%). The resting time between sampling points were further associated with minor changes in the total and CD4(+) rectal mucosal cell levels. The results add to general knowledge of in vivo evaluation of anti-HIV microbicide application concerning cellular effects in rectal mucosa.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
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Günaydin, Gökce
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Edfeldt, Gabriel ...
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Garber, David A.
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Asghar, Muhammad
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Noel-Romas, Laur ...
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Burgener, Adam
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visa fler...
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Wählby, Carolina ...
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Wang, Lin
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Rohan, Lisa C.
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Guenthner, Patri ...
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Mitchell, James
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Matoba, Nobuyuki
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McNicholl, Janet ...
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Palmer, Kenneth ...
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Tjernlund, Annel ...
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Broliden, Kristi ...
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Uppsala universitet
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Karolinska Institutet