Sökning: id:"swepub:oai:DiVA.org:uu-400679" > Impact of Q-Griffit...
Fältnamn | Indikatorer | Metadata |
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000 | 05434naa a2200541 4500 | |
001 | oai:DiVA.org:uu-400679 | |
003 | SwePub | |
008 | 200102s2019 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:142434748 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4006792 URI |
024 | 7 | a https://doi.org/10.1038/s41598-019-54493-42 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1424347482 URI |
040 | a (SwePub)uud (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Günaydin, Gökceu Karolinska Institutet4 aut |
245 | 1 0 | a Impact of Q-Griffithsin anti-HIV microbicide gel in non-human primates :b In situ analyses of epithelial and immune cell markers in rectal mucosa |
264 | c 2019-12-02 | |
264 | 1 | b NATURE PUBLISHING GROUP,c 2019 |
338 | a electronic2 rdacarrier | |
520 | a Natural-product derived lectins can function as potent viral inhibitors with minimal toxicity as shown in vitro and in small animal models. We here assessed the effect of rectal application of an anti-HIV lectin-based microbicide Q-Griffithsin (Q-GRFT) in rectal tissue samples from rhesus macaques. E-cadherin(+) cells, CD4(+) cells and total mucosal cells were assessed using in situ staining combined with a novel customized digital image analysis platform. Variations in cell numbers between baseline, placebo and Q-GRFT treated samples were analyzed using random intercept linear mixed effect models. The frequencies of rectal E-cadherin(+) cells remained stable despite multiple tissue samplings and Q-GRFT gel (0.1%, 0.3% and 1%, respectively) treatment. Whereas single dose application of Q-GRFT did not affect the frequencies of rectal CD4(+) cells, multi-dose Q-GRFT caused a small, but significant increase of the frequencies of intra-epithelial CD4(+) cells (placebo: median 4%; 1% Q-GRFT: median 7%) and of the CD4(+) lamina propria cells (placebo: median 30%; 0.1-1% Q-GRFT: median 36-39%). The resting time between sampling points were further associated with minor changes in the total and CD4(+) rectal mucosal cell levels. The results add to general knowledge of in vivo evaluation of anti-HIV microbicide application concerning cellular effects in rectal mucosa. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
700 | 1 | a Edfeldt, Gabriellau Karolinska Univ Hosp, Karolinska Inst, Ctr Mol Med, Dept Med Solna,Div Infect Dis, Stockholm, Sweden4 aut |
700 | 1 | a Garber, David A.u CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA4 aut |
700 | 1 | a Asghar, Muhammadu Karolinska Institutet4 aut |
700 | 1 | a Noel-Romas, Laurau Publ Hlth Agcy Canada, JC Wilt Infect Dis Res Ctr, Natl HIV & Retrovirol Labs, Winnipeg, MB, Canada;Univ Manitoba, Dept Obstet & Gynecol, Winnipeg, MB, Canada;Univ Manitoba, Dept Med Microbiol, Winnipeg, MB, Canada4 aut |
700 | 1 | a Burgener, Adamu Karolinska Institutet4 aut |
700 | 1 | a Wählby, Carolina,c professor,d 1974-u Uppsala universitet,Avdelningen för visuell information och interaktion,Science for Life Laboratory, SciLifeLab,Bildanalys och människa-datorinteraktion4 aut0 (Swepub:uu)cli05194 |
700 | 1 | a Wang, Linu Magee Womens Res Inst, Pittsburgh, PA USA4 aut |
700 | 1 | a Rohan, Lisa C.u Magee Womens Res Inst, Pittsburgh, PA USA;Univ Pittsburgh, Sch Pharm, Pittsburgh, PA USA4 aut |
700 | 1 | a Guenthner, Patriciau CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA4 aut |
700 | 1 | a Mitchell, Jamesu CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA4 aut |
700 | 1 | a Matoba, Nobuyukiu Univ Louisville, Ctr Predict Med, Louisville, KY 40292 USA;Univ Louisville, Dept Pharmacol & Toxicol, Louisville, KY 40292 USA;Univ Louisville, James Graham Brown Canc Ctr, Louisville, KY 40292 USA4 aut |
700 | 1 | a McNicholl, Janet M.u CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA4 aut |
700 | 1 | a Palmer, Kenneth E.u Univ Louisville, Ctr Predict Med, Louisville, KY 40292 USA;Univ Louisville, Dept Pharmacol & Toxicol, Louisville, KY 40292 USA;Univ Louisville, James Graham Brown Canc Ctr, Louisville, KY 40292 USA4 aut |
700 | 1 | a Tjernlund, Annelieu Karolinska Institutet4 aut |
700 | 1 | a Broliden, Kristinau Karolinska Institutet4 aut |
710 | 2 | a Karolinska Institutetb Karolinska Univ Hosp, Karolinska Inst, Ctr Mol Med, Dept Med Solna,Div Infect Dis, Stockholm, Sweden4 org |
773 | 0 | t Scientific Reportsd : NATURE PUBLISHING GROUPg 9q 9x 2045-2322 |
856 | 4 | u https://doi.org/10.1038/s41598-019-54493-4y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1382181/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 | u https://www.nature.com/articles/s41598-019-54493-4.pdf |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-400679 |
856 | 4 8 | u https://doi.org/10.1038/s41598-019-54493-4 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:142434748 |
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