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Transient Photoinac...
Transient Photoinactivation of Cell Membrane Protein Activity without Genetic Modification by Molecular Hyperthermia
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- Kang, Peiyuan (author)
- Univ Texas Dallas, Dept Mech Engn, 800 West Campbell Rd, Richardson, TX 75080 USA
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- Li, Xiaoqing (author)
- Univ Texas Dallas, Dept Bioengn, 800 West Campbell Rd, Richardson, TX 75080 USA
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- Liu, Yaning (author)
- Univ Texas Dallas, Dept Mech Engn, 800 West Campbell Rd, Richardson, TX 75080 USA
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- Shiers, Stephanie I. (author)
- Univ Texas Dallas, Sch Behav & Brain Sci, 800 West Campbell Rd, Richardson, TX 75080 USA
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- Xiong, Hejian (author)
- Univ Texas Dallas, Dept Mech Engn, 800 West Campbell Rd, Richardson, TX 75080 USA
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- Giannotta, Monica (author)
- FIRC Inst Mol Oncol IFOM, Vasc Biol Lab, I-20139 Milan, Italy
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- Dejana, Elisabetta (author)
- Uppsala universitet,Vaskulärbiologi,FIRC Inst Mol Oncol IFOM, Vasc Biol Lab, I-20139 Milan, Italy
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- Price, Theodore John (author)
- Univ Texas Dallas, Sch Behav & Brain Sci, 800 West Campbell Rd, Richardson, TX 75080 USA
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- Randrianalisoa, Jaona (author)
- Univ Reims, Inst Therm Mecan Mat ITheMM, EA 7548, F-51687 Reims 2, France
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- Nielsen, Steven O. (author)
- Univ Texas Dallas, Dept Chem & Biochem, 800 West Campbell Rd, Richardson, TX 75080 USA
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- Qin, Zhenpeng (author)
- Univ Texas Dallas, Dept Mech Engn, 800 West Campbell Rd, Richardson, TX 75080 USA;Univ Texas Dallas, Dept Bioengn, 800 West Campbell Rd, Richardson, TX 75080 USA;Univ Texas Southwestern Med Ctr Dallas, Dept Surg, 5323 Harry Hines Blvd, Dallas, TX 75390 USA
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(creator_code:org_t)
- 2019-10-15
- 2019
- English.
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In: ACS Nano. - : AMER CHEMICAL SOC. - 1936-0851 .- 1936-086X. ; 13:11, s. 12487-12499
- Related links:
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https://www.ncbi.nlm...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Precise manipulation of protein activity in living systems has broad applications in biomedical sciences. However, it is challenging to use light to manipulate protein activity in living systems without genetic modification. Here, we report a technique to optically switch off protein activity in living cells with high spatiotemporal resolution, referred to as molecular hyperthermia (MH). MH is based on the nanoscale-confined heating of plasmonic gold nanoparticles by short laser pulses to unfold and photoinactivate targeted proteins of interest. First, we show that protease-activated receptor 2 (PAR2), a G-protein-coupled receptor and an important pathway that leads to pain sensitization, can be photoinactivated in situ by MH without compromising cell proliferation. PAR2 activity can be switched off in laser-targeted cells without affecting surrounding cells. Furthermore, we demonstrate the molecular specificity of MH by inactivating PAR2 while leaving other receptors intact. Second, we demonstrate that the photoinactivation of a tight junction protein in brain endothelial monolayers leads to a reversible blood-brain barrier opening in vitro. Lastly, the protein inactivation by MH is below the nanobubble generation threshold and thus is predominantly due to the nanoscale heating. MH is distinct from traditional hyperthermia (that induces global tissue heating) in both its time and length scales: nanoseconds versus seconds, nanometers versus millimeters. Our results demonstrate that MH enables selective and remote manipulation of protein activity and cellular behavior without genetic modification.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- plasmonic nanoparticle
- nanosecond laser
- protein inactivation
- G-protein-coupled receptor
- blood-brain barrier
Publication and Content Type
- ref (subject category)
- art (subject category)
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ACS Nano
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- By the author/editor
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Kang, Peiyuan
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Li, Xiaoqing
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Liu, Yaning
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Shiers, Stephani ...
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Xiong, Hejian
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Giannotta, Monic ...
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Dejana, Elisabet ...
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Price, Theodore ...
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Randrianalisoa, ...
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Nielsen, Steven ...
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Qin, Zhenpeng
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
- Articles in the publication
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ACS Nano
- By the university
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Uppsala University