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Sökning: id:"swepub:oai:DiVA.org:uu-421086" > Impact of time and ...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005488naa a2200505 4500
001oai:DiVA.org:uu-421086
003SwePub
008201007s2020 | |||||||||||000 ||eng|
009oai:slubar.slu.se:107752
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4210862 URI
0247 a https://doi.org/10.1371/journal.pone.02369442 DOI
0247 a https://res.slu.se/id/publ/1077522 URI
040 a (SwePub)uud (SwePub)slu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Cunningham, Janetu Uppsala universitet,Ekselius: Psykiatri4 aut0 (Swepub:uu)janecunn
2451 0a Impact of time and temperature on gut microbiota and SCFA composition in stool samples
264 c 2020-08-03
264 1b PUBLIC LIBRARY SCIENCE,c 2020
338 a electronic2 rdacarrier
520 a Gut dysbiosis has been implicated in the pathophysiology of a growing number of non-communicable diseases. High through-put sequencing technologies and short chain fatty acid (SCFA) profiling enables surveying of the composition and function of the gut microbiota and provide key insights into host-microbiome interactions. However, a methodological problem with analyzing stool samples is that samples are treated and stored differently prior to submission for analysis potentially influencing the composition of the microbiota and its metabolites. In the present study, we simulated the sample acquisition of a large-scale study, in which stool samples were stored for up to two days in the fridge or at room temperature before being handed over to the hospital. To assess the influence of time and temperature on the microbial community and on SCFA composition in a controlled experimental setting, the stool samples of 10 individuals were exposed to room and fridge temperatures for 24 and 48 hours, respectively, and analyzed using 16S rRNA gene amplicon sequencing, qPCR and nuclear magnetic resonance spectroscopy. To best of our knowledge, this is the first study to investigate the influence of storage time and temperature on the absolute abundance of methanogens, and ofLactobacillus reuteri. The results indicate that values obtained for methanogens,L.reuteriand total bacteria are still representative even after storage for up to 48 hours at RT (20 degrees C) or 4 degrees C. The overall microbial composition and structure appeared to be influenced more by laboratory errors introduced during sample processing than by the actual effects of temperature and time. Although microbial activity was demonstrated by elevated SCFA at both 4 degrees C and RT, SCFAs ratios were more stable over the different conditions and may be considered as long as samples are come from similar storage conditions.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Gastroenterologi0 (SwePub)302132 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Gastroenterology and Hepatology0 (SwePub)302132 hsv//eng
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Mikrobiologi inom det medicinska området0 (SwePub)301092 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Microbiology in the medical area0 (SwePub)301092 hsv//eng
650 7a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng
7001 a Bramstång, Ludvigu Uppsala universitet,Ekselius: Psykiatri4 aut0 (Swepub:uu)ludbr572
7001 a Singh, Abhijeetu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Swedish Univ Agr Sci, BioCentrum, Dept Mol Sci, Uppsala, Sweden.4 aut
7001 a Jayarathna, Shishanthiu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Swedish Univ Agr Sci, BioCentrum, Dept Mol Sci, Uppsala, Sweden.4 aut
7001 a Rasmusson, Annicau Uppsala universitet,Ekselius: Psykiatri4 aut0 (Swepub:uu)annijaco
7001 a Moazzami, Aliu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Swedish Univ Agr Sci, BioCentrum, Dept Mol Sci, Uppsala, Sweden.4 aut0 (Swepub:slu)47697
7001 a Müller, Bettinau Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Swedish Univ Agr Sci, BioCentrum, Dept Mol Sci, Uppsala, Sweden.4 aut0 (Swepub:slu)48328
7102 a Uppsala universitetb Ekselius: Psykiatri4 org
7102 a Sveriges lantbruksuniversitet
7730 t PLOS ONEd : PUBLIC LIBRARY SCIENCEg 15:8q 15:8x 1932-6203
8564 u https://doi.org/10.1371/journal.pone.0236944y Fulltext
8564 u https://uu.diva-portal.org/smash/get/diva2:1473983/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 u https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0236944&type=printable
8564 u https://pub.epsilon.slu.se/id/eprint/17550/contentsx primaryx Raw objectx freey FULLTEXT
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-421086
8564 8u https://doi.org/10.1371/journal.pone.0236944
8564 8u https://res.slu.se/id/publ/107752

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