id:"swepub:oai:DiVA.org:uu-450102"
Search: id:"swepub:oai:DiVA.org:uu-450102" > Molecular character...
- 1 of 1
- Previous record
- Next record
- To hitlist
Molecular characterization of endangered Iranian terrestrial orchids using ISSR markers and association with floral and tuber-related phenotypic traits
-
- Gholami, Soheila (author)
- Univ Kurdistan, Fac Agr, Dept Hort Sci & Engn, Sanandaj 6617715175, Iran.
-
- Vafaee, Yavar (author)
- Univ Kurdistan, Fac Agr, Dept Hort Sci & Engn, Sanandaj 6617715175, Iran.;Univ Kurdistan, Med Plants Breeding & Dev Res Inst, Sanandaj 6617715175, Iran.
-
- Nazari, Farzad (author)
- Univ Kurdistan, Fac Agr, Dept Hort Sci & Engn, Sanandaj 6617715175, Iran.
- show more...
- show less...
- Univ Kurdistan, Fac Agr, Dept Hort Sci & Engn, Sanandaj 6617715175, Iran Univ Kurdistan, Fac Agr, Dept Hort Sci & Engn, Sanandaj 6617715175, Iran.;Univ Kurdistan, Med Plants Breeding & Dev Res Inst, Sanandaj 6617715175, Iran. (creator_code:org_t)
- 2021-01-16
- 2021
- English.
- In: PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS. - : Springer. - 0971-5894 .- 0974-0430. ; 27:1, s. 53-68
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- We aimed to study the genetic diversity, population structure, and phylogeny of Iranian orchids using inter-simple sequence repeat (ISSR) markers to find markers associated with phenotypic traits. Based on the phenotypic analysis, the inflorescence length and the flower number of studied accessions ranged from 3.92 to 27.13 cm and 5 to 50, respectively. On the other hand, the tuber length ranged from 1.80 to 9.35 cm. A total of 310 reproducible ISSR fragments with a size range of 150 to 3000 bp were amplified. ISSR primers provided an average polymorphism information content of 0.391, varied from 0.488 for UBC-876 to 0.351 for UBC-842. Os.J population showed the lowest genetic diversity (H = 0.057 and I = 0.075), while Oyst.JA population showed the highest genetic diversity (H = 0.114 and I = 0.158). At species level, the average coefficient of genetic differentiation (G(ST)) ranged from 0.265 for Orchis simia to 0.587 for Himantoglossum affine. Gene flow (Nm) varied from 1.38 (O. simia) to 0.756 (Anacamptis collina). The UPGMA genetic similarity dendrogram using Jaccard coefficients (r = 0.973) revealed six main clusters. Based on the Bayesian clustering method, the highest probability of the data was achieved when accessions were divided into eight groups. Floral and tuber-related phenotypic traits represented high correlations together, and they were associated with some ISSR bands based on the multiple association analysis. Altogether, ISSR markers proved to be useful for discrimination and clarification of the relationships among species and populations collected from geographically different locations. Furthermore, it could identify the polymorphism among accessions within each population and species.
Subject headings
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Genetik och förädling inom lantbruksvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Genetics and Breeding in Agricultural Sciences (hsv//eng)
Keyword
- Endangered orchids
- Genetic diversity
- ISSR
- Marker association
- Phylogeny
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Gholami, Soheila
- Vafaee, Yavar
- Nazari, Farzad
- Ghorbani, Abdolb ...
- About the subject
-
- AGRICULTURAL SCIENCES
- AGRICULTURAL SCI ...
- and Agricultural Bio ...
- and Genetics and Bre ...
- Articles in the publication
- PHYSIOLOGY AND M ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
