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First Immunoassay for Measuring Isoaspartate in Human Serum Albumin
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- Wang, Jijing (author)
- Karolinska Inst, Dept Med Biophys & Biochem, S-17177 Stockholm, Sweden.
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- Lundström, Susanna L. (author)
- Karolinska Institutet
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- Seelow, Sven (author)
- Karolinska Inst, Dept Med Biophys & Biochem, S-17177 Stockholm, Sweden.
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- Karolinska Institutet Karolinska Inst, Dept Med Biophys & Biochem, S-17177 Stockholm, Sweden (creator_code:org_t)
- 2021-11-05
- 2021
- English.
- In: Molecules. - : MDPI. - 1431-5157 .- 1420-3049. ; 26:21
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Isoaspartate (isoAsp) is a damaging amino acid residue formed in proteins mostly as a result of spontaneous deamidation of asparaginyl residues. An association has been found between isoAsp in human serum albumin (HSA) and Alzheimer’s disease (AD). Here we report on a novel monoclonal antibody (mAb) 1A3 with excellent specificity to isoAsp in the functionally important domain of HSA. Based on 1A3 mAb, an indirect enzyme-linked immunosorbent assay (ELISA) was developed, and the isoAsp occupancy in 100 healthy plasma samples was quantified for the first time, providing the average value of (0.74 ± 0.13)%. These results suggest potential of isoAsp measurements for supplementary AD diagnostics as well as for assessing the freshness of stored donor blood and its suitability for transfusion.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
Keyword
- in vitro diagnostics
- blood analysis
- monoclonal antibody (mAb)
- mass spectrometry
- enzyme-linked immunosorbent assay (ELISA)
- human serum albumin (HSA)
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