id:"swepub:oai:DiVA.org:uu-461720"
Sökning: id:"swepub:oai:DiVA.org:uu-461720" > A Genetically Encod...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04357naa a2200493 4500 | |
| 001 | oai:DiVA.org:uu-461720 | |
| 003 | SwePub | |
| 008 | 220208s2021 | |||||||||||000 ||eng| | |
| 009 | oai:prod.swepub.kib.ki.se:148283792 | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4617202 URI |
| 024 | 7 | a https://doi.org/10.3389/fchem.2021.7685352 DOI |
| 024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1482837922 URI |
| 040 | a (SwePub)uud (SwePub)ki | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Meineke, Birtheu Karolinska Institutet,Karolinska Inst, Dept Med Biochem & Biophys, Div Genome Biol, Sci Life Lab, Stockholm, Sweden.;Karolinska Inst, Ming Wai Lau Ctr Reparat Med, Stockholm Node, Stockholm, Sweden.4 aut |
| 245 | 1 0 | a A Genetically Encoded Picolyl Azide for Improved Live Cell Copper Click Labeling |
| 264 | c 2021-11-11 | |
| 264 | 1 | b Frontiers Media S.A.c 2021 |
| 338 | a electronic2 rdacarrier | |
| 520 | a Bioorthogonal chemistry allows rapid and highly selective reactivity in biological environments. The copper-catalyzed azide-alkyne cycloaddition (CuAAC) is a classic bioorthogonal reaction routinely used to modify azides or alkynes that have been introduced into biomolecules. Amber suppression is an efficient method for incorporating such chemical handles into proteins on the ribosome, in which noncanonical amino acids (ncAAs) are site specifically introduced into the polypeptide in response to an amber (UAG) stop codon. A variety of ncAA structures containing azides or alkynes have been proven useful for performing CuAAC chemistry on proteins. To improve CuAAC efficiency, biologically incorporated alkyne groups can be reacted with azide substrates that contain copper-chelating groups. However, the direct incorporation of copper-chelating azides into proteins has not been explored. To remedy this, we prepared the ncAA paz-lysine (PazK), which contains a picolyl azide motif. We show that PazK is efficiently incorporated into proteins by amber suppression in mammalian cells. Furthermore, PazK-labeled proteins show improved reactivity with alkyne reagents in CuAAC. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 653 | a genetic code expansion | |
| 653 | a amber suppression | |
| 653 | a noncanonical amino acid | |
| 653 | a bioorthogonal chemistry | |
| 653 | a click chemistry | |
| 653 | a copper catalyzed azide-alkyne cycloaddition (CuAAC) | |
| 700 | 1 | a Heimgärtner, Johannesu Karolinska Inst, Dept Med Biochem & Biophys, Div Genome Biol, Sci Life Lab, Stockholm, Sweden.;Karolinska Inst, Ming Wai Lau Ctr Reparat Med, Stockholm Node, Stockholm, Sweden.4 aut |
| 700 | 1 | a Craig, Alexander J.u Uppsala universitet,Läkemedelsdesign och läkemedelsutveckling4 aut0 (Swepub:uu)alecr417 |
| 700 | 1 | a Landreh, Michaelu Karolinska Institutet,Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Sci Life Lab, Stockholm, Sweden.4 aut |
| 700 | 1 | a Moodie, Lindon W. K.u Uppsala universitet,Läkemedelsdesign och läkemedelsutveckling,Uppsala antibiotikacentrum4 aut0 (Swepub:uu)linmo351 |
| 700 | 1 | a Elsässer, Simon J.u Karolinska Institutet,Karolinska Inst, Dept Med Biochem & Biophys, Div Genome Biol, Sci Life Lab, Stockholm, Sweden.;Karolinska Inst, Ming Wai Lau Ctr Reparat Med, Stockholm Node, Stockholm, Sweden.4 aut |
| 710 | 2 | a Karolinska Institutetb Karolinska Inst, Dept Med Biochem & Biophys, Div Genome Biol, Sci Life Lab, Stockholm, Sweden.;Karolinska Inst, Ming Wai Lau Ctr Reparat Med, Stockholm Node, Stockholm, Sweden.4 org |
| 773 | 0 | t Frontiers in Chemistryd : Frontiers Media S.A.g 9q 9x 2296-2646 |
| 856 | 4 | u https://doi.org/10.3389/fchem.2021.768535y Fulltext |
| 856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1635843/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
| 856 | 4 | u https://www.frontiersin.org/articles/10.3389/fchem.2021.768535/pdf |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-461720 |
| 856 | 4 8 | u https://doi.org/10.3389/fchem.2021.768535 |
| 856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:148283792 |
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