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Sökning: id:"swepub:oai:DiVA.org:uu-474373" > Evaluation of an ex...

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FältnamnIndikatorerMetadata
00004730naa a2200517 4500
001oai:DiVA.org:uu-474373
003SwePub
008220512s2022 | |||||||||||000 ||eng|
009oai:prod.swepub.kib.ki.se:235467176
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4743732 URI
0247 a https://doi.org/10.1186/s40635-022-00443-w2 DOI
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:2354671762 URI
040 a (SwePub)uud (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Skorup, Paulu Uppsala universitet,Infektionssjukdomar,Infektionsmedicin4 aut0 (Swepub:uu)pausk660
2451 0a Evaluation of an extracorporeal ozone-based bactericide system for the treatment of Escherichia coli sepsis
264 c 2022-04-25
264 1b Springer Nature,c 2022
338 a electronic2 rdacarrier
520 a Background: Sepsis is associated with substantial mortality rates. Antibiotic treatment is crucial, but global antibiotic resistance is now classified as one of the top ten global public health risks facing humanity. Ozone (O-3) is an inorganic molecule with no evident function in the body. We investigated the bactericide properties of ozone, using a novel system of extracorporeal ozone blood treatment. We hypothesized that ozone would decrease the concentration of viable Escherichia coli (E. coli) in human whole blood and that the system would be technically feasible and physiologically tolerable in a clinically relevant model of E. coli sepsis in swine. Methods: The E. coli strain B09-11822, a clinical isolate from a patient with septic shock was used. The in vitro study treated E. coli infected human whole blood (n = 6) with ozone. The in vivo 3.5-h sepsis model randomized swine to E. coli infusion and ozone treatment (n = 5) or E. coli infusion and no ozone treatment (n = 5). Live E. coli, 5 x 10(7) colony-forming units (CFU/mL) was infused in a peripheral vein. Ozone treatment was initiated with a duration of 30 min after 1.5 h. Results: The single pass in vitro treatment decreased E. coli by 27%, mean 1941 to 1422 CFU/mL, mean of differences - 519.0 (95% CI - 955.0 to - 82.98,P= 0.0281). pO(2) increased (95% CI 31.35 to 48.80, P= 0.0007), pCO(2) decreased (95% CI -3.203 to - 1.134, P= 0.0069), oxyhemoglobin increased (95% CI 1.010 to 3.669, P= 0.0113). Methemoglobin was not affected. In the sepsis model, 9/10 swine survived. One swine randomized to ozone treatment died from septic shock before initiation of the treatment. Circulatory, respiratory, and metabolic parameters were not affected by the ozone treatment. E. coli in arterial blood, in organs and in aerobic and anaerobic blood cultures did not differ. Hemoglobin, leucocytes, and methemoglobin were not affected by the treatment. Conclusions: Ozone decreased the concentration of viable E. coli in human whole blood. The system was technically feasible and physiologically tolerable in porcine sepsis/septic shock and should be considered for further studies towards clinical applications.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Infektionsmedicin0 (SwePub)302092 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Infectious Medicine0 (SwePub)302092 hsv//eng
653 a Porcine sepsis model
653 a Ozone
653 a E. coli
653 a Antibiotic resistance
653 a Intensive care
7001 a Fransson, Anetteu Karolinska Inst, Dept Neurosci, Sect Expt Traumatol, Biomed 8B, S-17177 Stockholm, Sweden.4 aut
7001 a Gustavsson, Jennyu Karolinska Institutet4 aut
7001 a Sjoholm, Johanu Sangair AB, Stockholm, Sweden.4 aut
7001 a Rundgren, Henriku Sangair AB, Stockholm, Sweden.4 aut
7001 a ozenci, Volkanu Karolinska Institutet4 aut
7001 a Wong, Alicia Y. W.u Karolinska Inst, Div Clin Microbiol, Dept Lab Med, Huddinge, Sweden.4 aut
7001 a Karlsson, Tomasu Karolinska Institutet4 aut
7001 a Svensen, Christeru Karolinska Institutet4 aut
7001 a Gunther, Mattiasu Karolinska Institutet4 aut
7102 a Uppsala universitetb Infektionssjukdomar4 org
7730 t Intensive Care Medicine Experimentald : Springer Natureg 10:1q 10:1x 2197-425X
8564 u https://doi.org/10.1186/s40635-022-00443-wy Fulltext
8564 u https://uu.diva-portal.org/smash/get/diva2:1657918/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-474373
8564 8u https://doi.org/10.1186/s40635-022-00443-w
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:235467176

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