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Rubisco forms a lat...
Rubisco forms a lattice inside alpha-carboxysomes
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- Metskas, Lauren Ann (författare)
- CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA.;Purdue Univ, Biol Sci Dept, W Lafayette, IN 47907 USA.;Purdue Univ, Chem Dept, W Lafayette, IN 47907 USA.
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- Ortega, Davi (författare)
- CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA.
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- Oltrogge, Luke M. (författare)
- Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA.
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- Blikstad, Cecilia (författare)
- Uppsala universitet,Institutionen för kemi - Ångström,Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA.
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- Lovejoy, Derik R. (författare)
- Purdue Univ, Biol Sci Dept, W Lafayette, IN 47907 USA.
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- Laughlin, Thomas G. (författare)
- Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA.;Univ Calif San Diego, Div Biol Sci, San Diego, CA 92103 USA.
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- Savage, David F. (författare)
- Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA.
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- Jensen, Grant J. (författare)
- CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA.;Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA.
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CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA;Purdue Univ, Biol Sci Dept, W Lafayette, IN 47907 USA.;Purdue Univ, Chem Dept, W Lafayette, IN 47907 USA. CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA. (creator_code:org_t)
- 2022-08-18
- 2022
- Engelska.
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Ingår i: Nature Communications. - : Springer Nature. - 2041-1723. ; 13:1
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Many autotrophic bacteria rely on Rubisco for carbon dioxide fixation. Here the authors report the position, orientation, and structure of Rubisco within alpha-carboxysomes; showing how it polymerizes and can form a lattice inside this compartment. Despite the importance of microcompartments in prokaryotic biology and bioengineering, structural heterogeneity has prevented a complete understanding of their architecture, ultrastructure, and spatial organization. Here, we employ cryo-electron tomography to image alpha-carboxysomes, a pseudo-icosahedral microcompartment responsible for carbon fixation. We have solved a high-resolution subtomogram average of the Rubisco cargo inside the carboxysome, and determined the arrangement of the enzyme. We find that the H. neapolitanus Rubisco polymerizes in vivo, mediated by the small Rubisco subunit. These fibrils can further pack to form a lattice with six-fold pseudo-symmetry. This arrangement preserves freedom of motion and accessibility around the Rubisco active site and the binding sites for two other carboxysome proteins, CsoSCA (a carbonic anhydrase) and the disordered CsoS2, even at Rubisco concentrations exceeding 800 mu M. This characterization of Rubisco cargo inside the alpha-carboxysome provides insight into the balance between order and disorder in microcompartment organization.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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