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Analysis of human l...
Analysis of human lung mast cells by single cell RNA sequencing
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- Ronnberg, Elin (author)
- Karolinska Inst, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.
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- Ravindran, Avinash (author)
- Karolinska Inst, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.;Karolinska Inst, Dept Med Huddinge, Ctr Infect Med, Stockholm, Sweden.
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- Mazzurana, Luca (author)
- Karolinska Inst, Dept Med Huddinge, Ctr Infect Med, Stockholm, Sweden.
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- Gong, Yitao (author)
- Karolinska Inst, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.
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- Safholm, Jesper (author)
- Karolinska Institutet,Karolinska Inst, Inst Environm Med, Res Ctr Allergy Res, Unit Expt Asthma & Allergy, Stockholm, Sweden.
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- Lorent, Julie, 1986- (author)
- Stockholms universitet,Science for Life Laboratory (SciLifeLab),Institutionen för biokemi och biofysik,Stockholm Univ, Sci Life Lab, Natl Bioinformat Infrastruct Sweden, Stockholm, Sweden.
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- Dethlefsen, Olga, 1983- (author)
- Stockholms universitet,Science for Life Laboratory (SciLifeLab),Institutionen för biokemi och biofysik,Stockholm Univ, Sci Life Lab, Natl Bioinformat Infrastruct Sweden, Stockholm, Sweden.
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- Orre, Ann-Charlotte (author)
- Karolinska Inst, Karolinska Univ Hosp, Dept Mol Med & Surg, Thorac Surg, Stockholm, Sweden.
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- Al-Ameri, Mamdoh (author)
- Karolinska Institutet,Karolinska Inst, Karolinska Univ Hosp, Dept Mol Med & Surg, Thorac Surg, Stockholm, Sweden.
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- Adner, Mikael (author)
- Karolinska Institutet,Karolinska Inst, Inst Environm Med, Res Ctr Allergy Res, Unit Expt Asthma & Allergy, Stockholm, Sweden.
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- Dahlen, Sven-Erik (author)
- Karolinska Inst, Inst Environm Med, Res Ctr Allergy Res, Unit Expt Asthma & Allergy, Stockholm, Sweden.
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- Dahlin, Joakim S. (author)
- Karolinska Inst, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.
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- Mjosberg, Jenny (author)
- Karolinska Inst, Dept Med Huddinge, Ctr Infect Med, Stockholm, Sweden.
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- Nilsson, Gunnar (author)
- Karolinska Institutet,Uppsala universitet,Hematologi,Karolinska Inst, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.
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Karolinska Inst, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden;Karolinska Univ Hosp, Stockholm, Sweden. Karolinska Inst, Dept Med Solna, Div Immunol & Allergy, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.;Karolinska Inst, Dept Med Huddinge, Ctr Infect Med, Stockholm, Sweden. (creator_code:org_t)
- 2023-03-30
- 2023
- English.
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In: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 14
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Abstract
Subject headings
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- Mast cells are tissue-resident cells playing major roles in homeostasis and disease conditions. Lung mast cells are particularly important in airway inflammatory diseases such as asthma. Human mast cells are classically divided into the subsets MCT and MCTC, where MCT express the mast cell protease tryptase and MCTC in addition express chymase, carboxypeptidase A3 (CPA3) and cathepsin G. Apart from the disctintion of the MCT and MCTC subsets, little is known about the heterogeniety of human lung mast cells and a deep analysis of their heterogeniety has previously not been performed. We therefore performed single cell RNA sequencing on sorted human lung mast cells using SmartSeq2. The mast cells showed high expression of classical mast cell markers. The expression of several individual genes varied considerably among the cells, however, no subpopulations were detected by unbiased clustering. Variable genes included the protease-encoding transcripts CMA1 (chymase) and CTSG (cathepsin G). Human lung mast cells are predominantly of the MCT subset and consistent with this, the expression of CMA1 was only detectable in a small proportion of the cells, and correlated moderately to CTSG. However, in contrast to established data for the protein, CPA3 mRNA was high in all cells and the correlation of CPA3 to CMA1 was weak.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- mast cells (MC)
- single cell RNA sequencing
- lung
- heterogeniety
- chymase (CMA1)
- cathepsin G (CTSG)
- carboxypeptidase A3 (CPA3)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Ronnberg, Elin
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Ravindran, Avina ...
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Mazzurana, Luca
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Gong, Yitao
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Safholm, Jesper
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Lorent, Julie, 1 ...
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show more...
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Dethlefsen, Olga ...
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Orre, Ann-Charlo ...
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Al-Ameri, Mamdoh
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Adner, Mikael
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Dahlen, Sven-Eri ...
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Dahlin, Joakim S ...
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Mjosberg, Jenny
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Nilsson, Gunnar
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Immunology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Frontiers in Imm ...
- By the university
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Uppsala University
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Stockholm University
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Karolinska Institutet