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Characterization of...
Characterization of immunoreactive dynorphin B and beta-endorphin in human plasma
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Silberring, Jerzy (author)
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Li, Yi-Ming (author)
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Terenius, Lars (author)
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- Nylander, Ingrid (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Pharmacology
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(creator_code:org_t)
- 1998
- 1998
- English.
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In: Peptides. - 0196-9781 .- 1873-5169. ; 19:8, s. 1329-1337
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Dynorphins and beta-endorphin in human plasma were characterized and studied quantitatively using radioimmunoassay, high-performance liquid chromatography (HPLC), and mass spectrometry. Most immunoreactive (ir) dynorphin B and beta-endorphin in human plasma coeluted with authentic peptides in analysis. Dynorphin A was not detected. Added to human plasma it was rapidly converted into Leu-enkephalin-Arg6 followed by elimination of the C-terminal arginine after prolonged incubation. The rate of dynorphin A conversion was estimated at 40 pmol/min/microl plasma. This process was inhibited by the thiol protease inhibitor, PHMB and by EDTA. Dynorphin B, alpha-neoendorphin and big dynorphin were virtually not metabolized by plasma proteases under the same conditions. beta-endorphin was processed into beta-endorphin(1-19) and the corresponding C-terminal counterpart beta-endorphin(20-31) at a rate of about 25 pmol/min/microl of plasma. Based on the above data, a reliable strategy was established to measure dynorphin B- and beta-endorphin-ir in human plasma samples. The basal levels in a male control group were 0.99 +/- 0.11 (n = 11) and 16.3 +/- 1.5 (n = 11) fmol/ml plasma, respectively.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Keyword
- dynorphins
- endorphins
- neuropeptides
- metabolism
- proteases
- protease inhibitors
- plasma
- human
- high pressure liquid chromatography
- mass spectrometry
- PHARMACY
- FARMACI
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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