id:"swepub:oai:DiVA.org:uu-50735"
Search: id:"swepub:oai:DiVA.org:uu-50735" > Characterization of...
- 1 of 1
- Previous record
- Next record
- To hitlist
Characterization of immunoreactive dynorphin B and beta-endorphin in human plasma
- Silberring, Jerzy (author)
- Li, Yi-Ming (author)
- Terenius, Lars (author)
- show more...
- show less...
- (creator_code:org_t)
- 1998
- 1998
- English.
- In: Peptides. - 0196-9781 .- 1873-5169. ; 19:8, s. 1329-1337
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Dynorphins and beta-endorphin in human plasma were characterized and studied quantitatively using radioimmunoassay, high-performance liquid chromatography (HPLC), and mass spectrometry. Most immunoreactive (ir) dynorphin B and beta-endorphin in human plasma coeluted with authentic peptides in analysis. Dynorphin A was not detected. Added to human plasma it was rapidly converted into Leu-enkephalin-Arg6 followed by elimination of the C-terminal arginine after prolonged incubation. The rate of dynorphin A conversion was estimated at 40 pmol/min/microl plasma. This process was inhibited by the thiol protease inhibitor, PHMB and by EDTA. Dynorphin B, alpha-neoendorphin and big dynorphin were virtually not metabolized by plasma proteases under the same conditions. beta-endorphin was processed into beta-endorphin(1-19) and the corresponding C-terminal counterpart beta-endorphin(20-31) at a rate of about 25 pmol/min/microl of plasma. Based on the above data, a reliable strategy was established to measure dynorphin B- and beta-endorphin-ir in human plasma samples. The basal levels in a male control group were 0.99 +/- 0.11 (n = 11) and 16.3 +/- 1.5 (n = 11) fmol/ml plasma, respectively.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Keyword
- dynorphins
- endorphins
- neuropeptides
- metabolism
- proteases
- protease inhibitors
- plasma
- human
- high pressure liquid chromatography
- mass spectrometry
- PHARMACY
- FARMACI
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Silberring, Jerz ...
- Li, Yi-Ming
- Terenius, Lars
- Nylander, Ingrid
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Pharmaceutical S ...
- Articles in the publication
- Peptides
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
