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Cryo-EM Map Anisotr...
Cryo-EM Map Anisotropy Can Be Attenuated by Map Post-Processing and a New Method for Its Estimation
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- Sanchez-Garcia, Ruben (författare)
- Univ Oxford, Dept Stat, 24-29 St Giles, Oxford OX1 3LB, England.
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- Gaullier, Guillaume (författare)
- Uppsala universitet,Molekylär systembiologi
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- Cuadra-Troncoso, Jose Manuel (författare)
- Univ Nacl Educ Distancia, Dept Inteligencia Artificial, C Juan del Rosal 16, Madrid 28040, Spain.
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- Vargas, Javier (författare)
- Univ Complutense Madrid, Dept Opt, Plaza Ciencias 1, Madrid 28040, Spain.
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Univ Oxford, Dept Stat, 24-29 St Giles, Oxford OX1 3LB, England Molekylär systembiologi (creator_code:org_t)
- MDPI, 2024
- 2024
- Engelska.
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Ingår i: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 25:7
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- One of the most important challenges in cryogenic electron microscopy (cryo-EM) is the substantial number of samples that exhibit preferred orientations, which leads to an uneven coverage of the projection sphere. As a result, the overall quality of the reconstructed maps can be severely affected, as manifested by the presence of anisotropy in the map resolution. Several methods have been proposed to measure the directional resolution of maps in tandem with experimental protocols to address the problem of preferential orientations in cryo-EM. Following these works, in this manuscript we identified one potential limitation that may affect most of the existing methods and we proposed an alternative approach to evaluate the presence of preferential orientations in cryo-EM reconstructions. In addition, we also showed that some of the most recently proposed cryo-EM map post-processing algorithms can attenuate map anisotropy, thus offering alternative visualization opportunities for cases affected by moderate levels of preferential orientations.
Ämnesord
- NATURVETENSKAP -- Data- och informationsvetenskap -- Datorseende och robotik (hsv//swe)
- NATURAL SCIENCES -- Computer and Information Sciences -- Computer Vision and Robotics (hsv//eng)
Nyckelord
- cryo-EM
- map anisotropy
- preferred orientations
- algorithms
- post-processing
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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