Sökning: id:"swepub:oai:DiVA.org:uu-53154" >
X-ray microanalysis...
X-ray microanalysis of in situ and isolated pancreatic islets
-
- Wroblewski, R (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
- Pålsgård, Eva (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
Juntti-Berggren, L (författare)
-
visa fler...
-
Berggren, P-O (författare)
-
- Roomans, G M (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
visa färre...
-
(creator_code:org_t)
- 1998
- 1998
- Engelska.
-
Ingår i: Pancreas. - 0885-3177 .- 1536-4828. ; 16:2, s. 134-140
- Relaterad länk:
-
https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- The effect of stimulation of insulin secretion in pancreatic beta cells on the elemental composition ofthese cells was investigated by x-ray microanalysis. In vitro experiments on isolated islets ofLangerhans from ob/ob mice were compared to in situ experiments. The only significant difference inthe elemental composition of beta cells from ob/ob mice versus their lean counterparts is a lower Ca concentration in the ob/ob animals. The nucleus of the beta cells has a higher concentration of P, K, and Na than the cytoplasm, which has a higher concentration of S and Cl. No polarized ion distributionin the cytoplasm of the beta cells was observed. Isolated beta cells show a higher concentration of Na and Cl and a lower concentration of K than their in situ counterparts. Stimulation of insulin secretion with glucose both in situ and in vitro showed only very small effects on the elemental composition of the beta cells: a tendency to a decreased P content was noted. In vitro experiments using stimulation with high extracellular K+ showed, in addition, a small increase in the intracellular K concentration. Inconclusion, while the elemental content of beta cells in vitro differs from that in situ, the response to glucose stimulation appears to be similar in both systems.
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
-
Pancreas
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas