Vitamin A metabolism and mRNA expression of retinoid-binding protein and receptor genes in human epidermal melanocytes and melanoma cells

Rosdahl, Inger (författare): Linköpings universitet,Dermatologi,Hälsouniversitetet

Kågedal, Bertil (författare): Linköpings universitet,Klinisk kemi,Hälsouniversitetet

Törmä, Hans (författare): Uppsala universitet,Institutionen för medicinska vetenskaper,Dermatology and Venereology,Department of Dermatology, University of Uppsala, Sweden

(creator_code:org_t)

Ovid Technologies (Wolters Kluwer Health), 1997
1997
Engelska.
Ingår i: Melanoma research. - : Ovid Technologies (Wolters Kluwer Health). - 0960-8931 .- 1473-5636. ; 7:4, s. 267-74

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Abstract Ämnesord

Stäng

Retinoids inhibit proliferation of melanocytes and melanoma cells and affect disorders of hypo- and hyperpigmentation. Such effects might involve retinoid-binding proteins, retinoid metabolites and nuclear retinoid receptors for transcriptional activation. We detected messenger RNA transcripts for the cellular retinol- and retinoic acid-binding proteins (CRBP, CRABP I and II) in cultured epidermal melanocytes. In the melanoma cell lines the major transcript was CRABP II. Nuclear retinoic acid (RA) receptor transcripts and the 9-cis-retinoic acid receptor transcript were detected in all cells. The endogenous concentrations of retinol (ROH) and its metabolite 3,4-didehydroretinol (ddROH) in melanocytes were five times those in melanoma cells. When cells were incubated with [3H]ROH the main metabolites in the melanocytes were [3H]ddROH (4%) and [3H]RA (0.4%). Formation of [3H]RA was only detected in one melanoma cell line. Both melanocytes and melanoma cells produced an unidentified metabolite when incubated with [3H]ROH and [3H]RA. Dissimilarities in the metabolism and endogenous concentration of retinoids between benign and malignant melanocytes might play a key role in differentiation and growth regulation.

Av författaren/redakt...: Rosdahl, Inger; Andersson, Eva; Kågedal, Bertil; Törmä, Hans

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