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Large-Scale Genotyp...
Large-Scale Genotyping for Analysis of the Type I Interferon System in Autoimmune Diseases
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- Sigurdsson, Snaevar, 1977- (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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Syvänen, Ann-Christine (thesis advisor)
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- Behrens, Timothy, Professor (opponent)
- Center for Immunology, Department of Medicine, University of Minnesota, Minneapolis
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(creator_code:org_t)
- ISBN 9155465323
- Uppsala : Acta Universitatis Upsaliensis, 2006
- English 82 s.
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Series: Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, 1651-6206 ; 138
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Abstract
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- Single nucleotide polymorphisms (SNPs) are the most common form of genetic variation. We developed a novel multiplexed method for SNP genotyping based on four-color fluorophore tag-microarray minisequencing. This method allows simultaneous genotyping of 80 samples and up to 200 SNPs in any allele combination. In study I we set up the method for a panel of SNPs from genes in the type I interferon system, and applied it in study III. In study II we used the technique to genotype SNPs from the coding region of the mitochondrial genome. A panel of 150 SNPs was genotyped in 265 individuals representing nine different populations. We demonstrated that the multiplexed SNP genotyping method for mitochondrial DNA increases the power of forensic identification in combination with sequencing of the hypervariable region of mitochondrial DNA. In study III we performed a genetic association study of SNPs in genes related to the type I Interferon system in Systemic Lupus Erythematosus (SLE). SLE is a chronic autoimmune inflammatory disease with a complex etiology. The SNPs were genotyped in DNA samples from Swedish, Finnish, and Icelandic patients with SLE, unaffected family members, and unrelated controls. The analysis identified SNPs in two genes, the tyrosine kinase 2 (TYK2) and interferon regulatory factor 5 (IRF5) genes that are highly associated with SLE with p-values <10-7 for joint linkage and association. Study IV describes the analysis of the TYK2 and IRF5 SNPs in a large Rheumatoid Arthritis (RA) sample cohort. We found that SNPs in the IRF5 gene were significantly associated with RA with a p-value = 0.00008. In contrast, we did not detect an association with SNPs in the TYK2 gene. These findings demonstrate that SLE and RA may have a common genetic background in the case of IRF5, while the TYK2 variants appear to be unique for SLE.
Keyword
- Molecular medicine
- Minisequencing
- SNP
- Microarray
- Polymorphism
- Mitochondria
- Systemic Lupus Erythematosus
- Rheumatoid Arthritis
- Association study
- Type I interferon
- Genotyping
- Molekylärmedicin
Publication and Content Type
- vet (subject category)
- dok (subject category)
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