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An optimistic view for quantifying mRNA in post-mortem human brain
- Preece, Paul (author)
- Virley, David J (author)
- Costandi, Moheb (author)
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- Coombes, Robert (author)
- Moss, Stephen J (author)
- Mudge, Anne W (author)
- Cairns, Nigel J (author)
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- (creator_code:org_t)
- 2003
- 2003
- English.
- In: Brain Research. Molecular Brain Research. - 0169-328X .- 1872-6941. ; 116:1-2, s. 7-16
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Quantitative human mRNA data are derived from post-mortem or biopsied tissue. RNA degradation, poor replication, a large mRNA variance and confounding factors such as brain pH and age of death are often cited, however, as objections to the data's reliability. A central question is whether post-mortem human mRNA can be treated as a statistically ordered system. TaqMan real-time RT-PCR was used to measure seven mRNAs in 513 cortical samples taken from 90 Alzheimer's disease and 81 control brains. Despite a high mRNA variance strong correlations were found between the mRNA transcripts in a single brain. Where a brain has a high/low level of one mRNA, the same brain invariably has a high/low level of other mRNAs; correlated order is present and allows removal of that source of variation common to all genes. Although levels of mRNA are highly variable between subjects (>1000-fold), quantitative order is present in post-mortem human mRNA, allowing effects due to pathology or gender to be isolated and tested for significance.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Keyword
- Aged
- Aged; 80 and over
- Alzheimer Disease/metabolism
- Amyloid beta-Protein Precursor/genetics
- Brain Chemistry
- Cadaver
- Case-Control Studies
- Comparative Study
- Female
- Glial Fibrillary Acidic Protein/genetics
- Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
- Humans
- Linear Models
- Male
- Microtubule-Associated Proteins/genetics
- Middle Aged
- Nerve Tissue Proteins/genetics
- Peptide Fragments/genetics
- Postmortem Changes
- RNA Stability
- RNA; Messenger/*analysis/chemistry
- Reproducibility of Results
- Reverse Transcriptase Polymerase Chain Reaction/methods
- Sex Factors
- Taq Polymerase
- Neurobiology
- Neurobiologi
- Genetics
- Genetik
Publication and Content Type
- ref (subject category)
- art (subject category)
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- Preece, Paul
- Virley, David J
- Costandi, Moheb
- Coombes, Robert
- Moss, Stephen J
- Mudge, Anne W
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- Jazin, Elena
- Cairns, Nigel J
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Neurosciences
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- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Genetics
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- Brain Research. ...
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- Uppsala University
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