Sökning: id:"swepub:oai:DiVA.org:uu-82749" > Development of real...
Fältnamn | Indikatorer | Metadata |
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000 | 03617naa a2200421 4500 | |
001 | oai:DiVA.org:uu-82749 | |
003 | SwePub | |
008 | 080625s2006 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-827492 URI |
024 | 7 | a https://doi.org/10.1016/j.jviromet.2006.04.0052 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Muradrasoli, Shamanu Uppsala universitet,Institutionen för medicinska vetenskaper,Blomberg4 aut0 (Swepub:uu)shamu791 |
245 | 1 0 | a Development of real-time PCRs for detection and quantitation of human MMTV-Iike (HML) sequences HML expression in human tissues |
264 | 1 | b Elsevier BV,c 2006 |
338 | a print2 rdacarrier | |
520 | a The human genome contains around 1000 betaretrovirus-like copies, human mouse mammary tumour virus (MMTV)-like (HML) groups 1 - 10, also referred to as human endogenous retroviruts "HERV-K". Despite many efforts, it is not established whether betaretroviruses, exo- or endogenous, are involved in the etiology of breast cancer, or other cancer diseases, in humans. Quantitative real-time PCR (QPCR) TaqMan((R))-based assays for HML groups 1-7, targeting the conserved reverse transcriptase (RT) and integrase (IN) domains of the pol gene were designed. Plasmids containing the entire pol gene of HML 1-7 were used as standards. The RT and IN based QPCRs could detect 10(0)-10(3) copies per PCR reaction of the plasmids. However, not all plasmids gave a signal in both RT and IN QPCRs, probably due to mismatches. Furthermore, RT and IN based HML6 specific QPCRs were developed. They were specific for amplification of transcripts for the whole HML6 group. The methods allow the monitoring in body fluids and tissues of expression of a wide range of betaretrovirus-like sequences. Betaretrovirus-like RNA was studied in normal human tissues and of HML6 in brains of multiple sclerosis (MS) patients. Brain, adrenal gland and testis had a high betaretrovirus-like expression. Multiple sclerosis plaques contained the same HML6 RNA concentration as control tissue. These assays are expected to enhance studies on involvement of betaretroviruses in physiology and disease. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Mikrobiologi inom det medicinska området0 (SwePub)301092 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Microbiology in the medical area0 (SwePub)301092 hsv//eng |
653 | a HML | |
653 | a endogenous retrovirus | |
653 | a betaretrovirus | |
653 | a real-time PCR | |
653 | a expression | |
653 | a transcription | |
653 | a Clinical virology | |
653 | a Klinisk virologi | |
700 | 1 | a Forsman, Annau Uppsala universitet,Institutionen för medicinska vetenskaper,Blomberg4 aut |
700 | 1 | a Hu, Lijuanu Uppsala universitet,Institutionen för medicinska vetenskaper,Blomberg4 aut0 (Swepub:uu)lihux020 |
700 | 1 | a Blikstad, Vidaru Uppsala universitet,Institutionen för medicinska vetenskaper,Blomberg4 aut0 (Swepub:uu)vibli031 |
700 | 1 | a Blomberg, Jonasu Uppsala universitet,Institutionen för medicinska vetenskaper,Blomberg4 aut0 (Swepub:uu)jonablom |
710 | 2 | a Uppsala universitetb Institutionen för medicinska vetenskaper4 org |
773 | 0 | t Journal of Virological Methodsd : Elsevier BVg 136:1-2, s. 83-92q 136:1-2<83-92x 0166-0934x 1879-0984 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-82749 |
856 | 4 8 | u https://doi.org/10.1016/j.jviromet.2006.04.005 |
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