id:"swepub:oai:DiVA.org:uu-97678"
Search: id:"swepub:oai:DiVA.org:uu-97678" > Increased striatal ...
- 1 of 1
- Previous record
- Next record
- To hitlist
Increased striatal mRNA and protein levels of the immunophilin FKBP-12 in experimental Parkinson’s Disease and identification of FKBP-12-binding proteins
-
- Nilsson, Anna (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
-
- Sköld, Karl (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
- Sjögren, Benita (author)
- show more...
- Caprioli, Richard M. (author)
- Buijs, Jos (author)
- Persson, Björn (author)
- show less...
- (creator_code:org_t)
- 2007-09-18
- 2007
- English.
- In: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 6:10, s. 3952-3961
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- FKBP-12, a 12 kDa FK506-binding protein (neuroimmunophilin), acts as a receptor for the immunosuppressant drug FK506. Neuroimmunophilins, including FKBP-12, are abundant in the brain and have been shown to be involved in reversing neuronal degeneration and preventing cell death. In this report, we have utilized several analytical techniques, such as in situ hybridization, Western blotting, two-dimensional gel electrophoresis, and liquid chromatography electrospray tandem mass spectrometry to study the transcriptional expression as well as protein levels of FKBP-12 in the unilateral 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease. The FKBP-12 protein was also detected directly on brain tissue sections using mass spectrometry profiling. We found increased levels of FKBP-12 mRNA and protein in the dorsal and middle part of the 6-OHDA lesioned striatum. Thus, these studies clearly demonstrate that FKBP-12 is increased in the brain of a common animal model of Parkinson's disease (PD). Additionally, we have identified potential binding partners to FKBP-12 that may be implicated in the pathophysiology of Parkinson's disease, such as alpha-enolase, 14-3-3 zeta/delta, pyruvate kinase isozymes, and heat shock protein 70, using surface plasmon resonance sensor technology in combination with mass spectrometry. In conclusion, these data strongly suggests that FKBP-12 is altered in an experimental model of PD.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
Keyword
- Parkinson's disease
- proteomics
- protein interactions
- 6-OHDA
- striatum
- mass spectrometry
- PHARMACY
- FARMACI
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Journal of Proteome Research (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Nilsson, Anna
- Sköld, Karl
- Sjögren, Benita
- Svensson, Marcus
- Pierson, Johan
- Zhang, Xiaoqun
- show more...
- Caprioli, Richar ...
- Buijs, Jos
- Persson, Björn
- Svenningsson, Pe ...
- Andrén, Per E.
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Pharmaceutical S ...
- Articles in the publication
- Journal of Prote ...
- By the university
- Uppsala University
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
