Blocking peptides decrease tissue transglutaminase processing of gliadin in vitro

Hoffmann, Karolina, 1980 (author): Chalmers tekniska högskola,Chalmers University of Technology

Alminger, Marie, 1957 (author): Chalmers tekniska högskola,Chalmers University of Technology

Andlid, Thomas, 1963 (author): Chalmers tekniska högskola,Chalmers University of Technology

Olsson, Olof, 1950 (author): Gothenburg University,Göteborgs universitet,Institutionen för växt- och miljövetenskaper,Department of Plant and Environmental Sciences,University of Gothenburg

Sandberg, Ann-Sofie, 1951 (author): Chalmers tekniska högskola,Chalmers University of Technology

(creator_code:org_t)

American Chemical Society (ACS), 2009
2009
English.
In: Journal of Agricultural and Food Chemistry. - : American Chemical Society (ACS). - 0021-8561 .- 1520-5118. ; 57:21, s. 10150-10155

Related links:: http://dx.doi.org/10...; show more...; https://gup.ub.gu.se...; https://doi.org/10.1...; https://research.cha...; show less...

Abstract Subject headings

Tissue transglutaminase (tTG) plays an important role in celiac disease pathology as it catalyzes deamidation and cross-linking of specific gluten peptides and converts them into potent epitopes recognized by intestinal T-cells. We investigated whether synthetic peptides with high affinity to gliadin could alter tTG activity on gliadin and whole gluten digest. The immobilized substrates were incubated with synthetic peptides identified by the phage display technique and a control peptide with no affinity to gliadin. Transglutaminase activity was measured with time resolved fluorescence. The mean tTG activity, compared to that of the control without the peptides, was reduced by 31, 33, and 36% for three selected gliadin-binding peptides, and 30% for the peptide pool (P <= 0.001-0.004) when gliadin was the substrate. Finally, substrate specificity experiments suggested that avenin was processed in a manner similar that used for gliadin during in vitro assays with tTG. The results showed that the blocking peptides efficiently reduced tTG processing of gliadin in vitro, and this strategy will be further investigated as an alternative therapy for celiac disease.

By the author/editor: Hoffmann, Karoli ...; Alminger, Marie, ...; Andlid, Thomas, ...; Chen, Tingsu; Olsson, Olof, 19 ...; Sandberg, Ann-So ...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Immunology in th ...

By the university: University of Gothenburg; Chalmers University of Technology

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