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Post-translational fate of a mucin-like leukocyte sialoglycoprotein (CD43) aberrantly expressed in a colon carcinoma cell line.
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- Baeckström, Dan, 1956 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
- (creator_code:org_t)
- 1997
- 1997
- English.
- In: The Journal of biological chemistry. - 0021-9258. ; 272:17, s. 11503-9
- Journal article (peer-reviewed)
Abstract
Subject headings
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- This paper describes the biosynthesis of L-CanAg, a mucin-like glycoprotein which carries the carcinoma-associated carbohydrate epitope sialyl-Lewis a and is secreted by the colon adenocarcinoma cell line COLO 205. Recently, it has been shown that L-CanAg is a novel glycoform of CD43, a surface sialoglycoprotein normally found only on hematopoietic cells. Immunoprecipitation with alpha-GPEP18, a novel antiserum against the cytoplasmic domain of CD43, detected a transmembrane form of L-CanAg carrying sialyl-Lewis a. Cell surface biotinylation experiments demonstrated the presence of transmembrane L-CanAg at the plasma membrane and that COLO 205, unlike the leukocyte cell line HL-60, contained significant amounts of glycosylated intracellular CD43. Immunoprecipitation of phosphate-labeled COLO 205 cells revealed that membrane-bound L-CanAg, like leukocyte CD43, is a phosphoprotein. Interestingly, both surface- and phosphate-labeled L-CanAg were eluted earlier from a gel filtration column than their unlabeled counterparts, indicating that this method could separate membrane-bound L-CanAg from its soluble form. Immunoprecipitations of pulse-chase-labeled COLO 205 lysates fractionated by gel filtration showed that decrease in membrane-bound L-CanAg was concomitant with an increase in the intracellular soluble form. Together, these data indicate that transmembrane L-CanAg is fully glycosylated and phosphorylated before the extracellular domain is cleaved off and stored inside the cell before exocytosis.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Adenocarcinoma
- metabolism
- Antigens
- CD
- biosynthesis
- immunology
- Antigens
- CD43
- Antigens
- Tumor-Associated
- Carbohydrate
- biosynthesis
- immunology
- Carbohydrate Sequence
- Colonic Neoplasms
- metabolism
- Gangliosides
- biosynthesis
- immunology
- Glycosylation
- HL-60 Cells
- Humans
- Leukocytes
- Membrane Proteins
- biosynthesis
- Molecular Sequence Data
- Mucins
- metabolism
- Phosphoproteins
- biosynthesis
- immunology
- Phosphorylation
- Protein Processing
- Post-Translational
- Sialoglycoproteins
- biosynthesis
- immunology
- Tumor Cells
- Cultured
Publication and Content Type
- ref (subject category)
- art (subject category)
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