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A novel non-toxic c...
A novel non-toxic combined CTA1-DD and ISCOMS adjuvant vector for effective mucosal immunization against influenza virus.
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- Grdic Eliasson, Dubravka (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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Helgeby, Anja, 1967 (author)
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- Schön, Karin, 1962 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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Nygren, Caroline (author)
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El-Bakkouri, Karim (author)
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Fiers, Walter (author)
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Saelens, Xavier (author)
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Lövgren, Karin Bengtsson (author)
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Nyström, Ida (author)
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- Lycke, Nils Y, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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(creator_code:org_t)
- Elsevier BV, 2011
- 2011
- English.
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In: Vaccine. - : Elsevier BV. - 1873-2518 .- 0264-410X. ; 29:23, s. 3951-61
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https://doi.org/10.1...
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Abstract
Subject headings
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- Here we demonstrate that by using non-toxic fractions of saponin combined with CTA1-DD we can achieve a safe and above all highly efficacious mucosal adjuvant vector. We optimized the construction, tested the requirements for function and evaluated proof-of-concept in an influenza A virus challenge model. We demonstrated that the CTA1-3M2e-DD/ISCOMS vector provided 100% protection against mortality and greatly reduced morbidity in the mouse model. The immunogenicity of the vector was superior to other vaccine formulations using the ISCOM or CTA1-DD adjuvants alone. The versatility of the vector was best exemplified by the many options to insert, incorporate or admix vaccine antigens with the vector. Furthermore, the CTA1-3M2e-DD/ISCOMS could be kept 1 year at 4°C or as a freeze-dried powder without affecting immunogenicity or adjuvanticity of the vector. Strong serum IgG and mucosal IgA responses were elicited and CD4 T cell responses were greatly enhanced after intranasal administration of the combined vector. Together these findings hold promise for the combined vector as a mucosal vaccine against influenza virus infections including pandemic influenza. The CTA1-DD/ISCOMS technology represents a breakthrough in mucosal vaccine vector design which successfully combines immunomodulation and targeting in a safe and stable particulate formation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Adjuvants
- Immunologic
- Animals
- Cholera Toxin
- administration & dosage
- genetics
- immunology
- Genetic Vectors
- administration & dosage
- immunology
- Humans
- ISCOMs
- administration & dosage
- genetics
- immunology
- Immunity
- Mucosal
- Immunization
- Influenza A Virus
- H1N1 Subtype
- immunology
- pathogenicity
- Influenza A Virus
- H3N2 Subtype
- immunology
- pathogenicity
- Influenza Vaccines
- administration & dosage
- genetics
- immunology
- Mice
- Mice
- Inbred BALB C
- Mice
- Inbred C57BL
- Mice
- Transgenic
- Mucous Membrane
- immunology
- Orthomyxoviridae Infections
- immunology
- prevention & control
- virology
- Reassortant Viruses
- immunology
- pathogenicity
- Recombinant Fusion Proteins
- administration & dosage
- genetics
- immunology
- Treatment Outcome
- Viral Matrix Proteins
- administration & dosage
- genetics
- immunology
Publication and Content Type
- ref (subject category)
- art (subject category)
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Vaccine
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Grdic Eliasson, ...
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Helgeby, Anja, 1 ...
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Schön, Karin, 19 ...
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Nygren, Caroline
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El-Bakkouri, Kar ...
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Fiers, Walter
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Saelens, Xavier
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Lövgren, Karin B ...
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Nyström, Ida
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Lycke, Nils Y, 1 ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
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Vaccine
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University of Gothenburg