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Searching for Mechanisms of N-Methyl-D-Aspartate-Induced Glutathione Efflux in Organotypic Hippocampal Cultures
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- Wallin, Camilla, 1968 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Abbas, Abdul-Karim, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Tranberg, Mattias, 1977 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för medicinsk fysik,Institute of Physiology and Pharmacology, Dept of Medical Biophysics
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Weber, Stephen G. (author)
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- Wigström, Holger, 1946 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för medicinsk fysik,Institute of Physiology and Pharmacology, Dept of Medical Biophysics
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- Sandberg, Mats, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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(creator_code:org_t)
- 2003
- 2003
- English.
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In: Neurochemical Research. - 0364-3190. ; 28:2, s. 281-291
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Abstract
Subject headings
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- N-Methyl-D-aspartate (NMDA)-receptor stimulation evoked a selective and partly delayed elevated efflux of glutathione, phosphoethanolamine, and taurine from organotypic rat hippocampus slice cultures. The protein kinase inhibitors H9 and staurosporine had no effect on the efflux. The phospholipase A2 inhibitors quinacrine and 4-bromophenacyl bromide, as well as arachidonic acid, a product of phospholipase A2 activity, did not affect the stimulated efflux. Polymyxin B, an antimicrobal agent that inhibits protein kinase C, and quinacrine in high concentration (500 microM), blocked efflux completely. The stimulated efflux after but not during NMDA incubation was attenuated by a calmodulin antagonist (W7) and an anion transport inhibitor (DNDS). Omission of calcium increased the spontaneous efflux with no or small additional effects by NMDA. In conclusion, NMDA receptor stimulation cause an increased selective efflux of glutathione, phosphoethanolamine and taurine in organotypic cultures of rat hippocampus. The efflux may partly be regulated by calmodulin and DNDS sensitive channels.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- NMDA; glutathione; phosphoethanolamine; taurine; efflux
Publication and Content Type
- ref (subject category)
- art (subject category)
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