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Life-long in vivo c...
Life-long in vivo cell-lineage tracing shows that no oogenesis originates from putative germline stem cells in adult mice
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- Zhang, Hua (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Liu, L. (author)
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- Li, Xin (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Busayavalasa, Kiran (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Shen, Yan (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Hovatta, O. (author)
- Karolinska Institutet
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- Gustafsson, J. A. (author)
- Karolinska Institutet
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- Liu, Kui (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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(creator_code:org_t)
- 2014-12
- 2014
- English.
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In: Proceedings of the National Academy of Science of the United States of America. - : National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 111:50, s. 17983-17988
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Abstract
Subject headings
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- Whether or not oocyte regeneration occurs in adult life has been the subject of much debate. In this study, we have traced germcell lineages over the life spans of three genetically modified mouse models and provide direct evidence that oogenesis does not originate from any germline stem cells (GSCs) in adult mice. By selective ablation of all existing oocytes in a Gdf9-Cre;iDTR mouse model, we have demonstrated that no new germ cells were ever regenerated under pathological conditions. By in vivo tracing of oocytes and follicles in the Sohlh1-CreERT2;R26R and Foxl2-CreERT2;mT/mG mouse models, respectively, we have shown that the initial pool of oocytes is the only source of germ cells throughout the life span of the mice and that no adult oogenesis ever occurs under physiological conditions. Our findings clearly show that there are no GSCs that contribute to adult oogenesis in mice and that the initial pool of oocytes formed in early life is the only source of germ cells throughout the entire reproductive life span.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Follicle tracing
- Genetically modified mouse models
- Life-long observations
- No postnatal oocyte regeneration
- Oocyte tracing
- cre recombinase
- growth differentiation factor 9
- messenger RNA
- adult
- animal cell
- animal experiment
- animal tissue
- Article
- cell lineage
- controlled study
- corpus luteum
- female
- gene expression
- genetically engineered mouse strain
- germline stem cell
- lifespan
- male
- mouse
- nonhuman
- oocyte development
- ovary
- primordial germ cell
- regeneration
- reverse transcription polymerase chain reaction
- somatic cell
- spermatogenesis
- Mus
Publication and Content Type
- ref (subject category)
- art (subject category)
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