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Single-molecule ima...
Single-molecule imaging of individual amyloid protein aggregates in human biofluids.
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Horrocks, Mathew Harry (author)
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Lee, Steven F (author)
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Gandhi, Sonia (author)
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Magdalinou, Nadia K (author)
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Chen, Serene W (author)
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Devine, Michael J (author)
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Tosatto, Laura (author)
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Kjaergaard, Magnus (author)
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Beckwith, Joseph S (author)
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- Zetterberg, Henrik, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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Iljina, Marija (author)
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Cremades, Nunilo (author)
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Dobson, Christopher M (author)
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Wood, Nicholas W (author)
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Klenerman, David (author)
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(creator_code:org_t)
- 2016-02-04
- 2016
- English.
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In: ACS chemical neuroscience. - : American Chemical Society (ACS). - 1948-7193. ; 16;7:3, s. 399-406
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
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- The misfolding and aggregation of proteins into amyloid fibrils characterizes many neurodegenerative disorders such as Parkinson's and Alzheimer's diseases. We report here a method, termed SAVE (single aggregate visualization by enhancement) imaging, for the ultra-sensitive detection of individual amyloid fibrils and oligomers using single-molecule fluorescence microscopy. We demonstrate that this method is able to detect the presence of amyloid aggregates of alpha-synuclein, tau and amyloid-β. In addition, we show that aggregates can also be identified in human cerebrospinal fluid (CSF). Significantly, we see a two-fold increase in the average aggregate concentration in CSF from PD patients compared to age-matched controls. Taken together, we conclude that this method provides an opportunity to characterize the structural nature of amyloid aggregates in a key biofluid, and therefore has the potential to study disease progression in both animal models and humans to enhance our understanding of neurodegenerative disorders.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Horrocks, Mathew ...
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Lee, Steven F
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Gandhi, Sonia
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Magdalinou, Nadi ...
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Chen, Serene W
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Devine, Michael ...
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show more...
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Tosatto, Laura
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Kjaergaard, Magn ...
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Beckwith, Joseph ...
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Zetterberg, Henr ...
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Iljina, Marija
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Cremades, Nunilo
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Dobson, Christop ...
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Wood, Nicholas W
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Klenerman, David
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- Articles in the publication
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ACS chemical neu ...
- By the university
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University of Gothenburg