Sperm Accumulated Against Surface: A novel alternative bioassay for environmental monitoring

Forecasting the impacts of changes in water quality on broadcast spawning aquatic organisms is a key aspect of environmental monitoring. Rapid assays of reproductive potential are central to this monitoring, and there is a need to develop a variety of methods to identify responses. Here, we report a proof of-concept study that assesses whether quantification of "Sperm Accumulated Against Surface" (SAAS) of tissue culture well-plates could be a rapid and simple proxy measure of fertilisation success. Our results confirm that motile sperm (but not immotile sperm) actively accumulate at surfaces and that the pattern of accumulation reflects fertilisation success in the model oyster species Crassostrea gigas. Furthermore, we confirm these patterns of SAAS for another marine species, the polychaete Galeolaria caespitosa, as well as for a freshwater species, the fish Gasterosteus aculeatus. For all species considered, SAAS reflected changes in sperm performance caused by experimentally manipulated differences in water quality (here, salinity). These findings indicate that SAAS could be applied easily to a range of species when examining the effects of water quality. Measurement of SAAS could, therefore, form the basis of a rapid and reliable assay for bioassessments of broadcast spawning aquatic organisms. (C) 2015 Elsevier Ltd. All rights reserved.

NATURVETENSKAP Geovetenskap och miljövetenskap Miljövetenskap hsv//swe
NATURAL SCIENCES Earth and Related Environmental Sciences Environmental Sciences hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Farmakologi och toxikologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Pharmacology and Toxicology hsv//eng
NATURVETENSKAP Biologi Ekologi hsv//swe
NATURAL SCIENCES Biological Sciences Ecology hsv//eng
Bioassay
Bioassessment
Ecotoxicology
Oyster
Polychaete
Sperm movement
Spermatozoa
reproductive end-points
near-future levels
cod gadus-morhua
ocean
acidification
analysis casa
sea-urchin
fertilization kinetics
salinity
motility
marine
Environmental Sciences & Ecology
Marine & Freshwater Biology
Toxicology

Havenhand, Jonathan N.,1959Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap, Tjärnö marinbiologiska laboratorium,Department of Biological and Environmental Sciences, Tjärnö Marine Biological Laboratory(Swepub:gu)xhavjo (författare)
Styan, C. A. (författare)
Göteborgs universitetInstitutionen för biologi och miljövetenskap, Tjärnö marinbiologiska laboratorium (creator_code:org_t)

Av författaren/redakt...: Falkenberg, L. J ...; Havenhand, Jonat ...; Styan, C. A.

Om ämnet

NATURVETENSKAP: NATURVETENSKAP; och Geovetenskap och ...; och Miljövetenskap

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Farmakologi och ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.