Precursor B Cells Increase in the Lung during Airway Allergic Inflammation: A Role for B Cell-Activating Factor

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Precursor B Cells Increase in the Lung during Airway Allergic Inflammation: A Role for B Cell-Activating Factor

Samitas, Konstantinos, 1977 (author): Gothenburg University,Göteborgs universitet,Krefting Research Centre

Malmhäll, Carina, 1959 (author): Gothenburg University,Göteborgs universitet,Krefting Research Centre

Rådinger, Madeleine, 1967 (author): Gothenburg University,Göteborgs universitet,Krefting Research Centre

Ramos-Ramírez, Patricia (author): Gothenburg University,Göteborgs universitet,Krefting Research Centre

Lu, You, 1982 (author): Gothenburg University,Göteborgs universitet,Krefting Research Centre

Deák, Tünde, 1983 (author): Gothenburg University,Göteborgs universitet,Krefting Research Centre

Semitekolou, M. (author)

Gaga, M. (author)

Sjöstrand, Margareta, 1947 (author): Gothenburg University,Göteborgs universitet,Krefting Research Centre

Lötvall, Jan, 1956 (author): Gothenburg University,Göteborgs universitet,Krefting Research Centre

Bossios, Apostolos, 1969 (author): Gothenburg University,Göteborgs universitet,Krefting Research Centre

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2016-08-11
2016
English.
In: Plos One. - : Public Library of Science (PLoS). - 1932-6203. ; 11:8

Related links:: https://doi.org/10.1...; show more...; https://gup.ub.gu.se...; https://doi.org/10.1...; http://kipublication...; show less...

Journal article (peer-reviewed)

Abstract Subject headings

Background B cells, key cells in allergic inflammation, differentiate in the bone marrow and their precursors include pro-B, pre-B and immature B cells. Eosinophil progenitor cells increase in the lung after allergen exposure. However, the existence and possible role of B cell precursors in the lung during allergic inflammation remains elusive. A BALB/c mouse model of allergic airway inflammation was utilized to perform phenotypic and quantification analyses of pro-B and pre-B cells in the lung by flow cytometry. B cell maturation factors IL-7 and B cell-activating factor (BAFF) and their receptors (CD127 and BAFFR, BCMA, TACI, respectively) were also evaluated in the lung and serum. The effect of anti-BAFF treatment was investigated both in vivo (i.p. administration of BAFF-R-Ig fusion protein) and in vitro (colony forming cell assay). Finally, BAFF levels were examined in the bronchoalveolar lavage (BAL) of asthmatic patients and healthy controls. Precursor pro and pre-B cells increase in the lung after allergen exposure, proliferate in the lung tissue in vivo, express markers of chemotaxis (CCR10 and CXCR4) and co-stimulation (CD40, CD86) and are resistant to apoptosis (Bax). Precursor B cells express receptors for BAFF at baseline, while after allergen challenge both their ligand BAFF and the BCMA receptor expression increases in B cell precursors. Blocking BAFFR in the lung in vivo decreases eosinophils and proliferating precursor B cells. Blocking BAFFR in bone marrow cultures in vitro reduces pre-B colony formation units. BAFF is increased in the BAL of severe asthmatics. Our data support the concept of a BAFF-mediated role for B cell precursors in allergic airway inflammation.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)

Keyword

bone-marrow
eosinophil progenitors
ccr10 expression
secreting cells
factor baff
asthma
survival
mice
differentiation
proliferation
Science & Technology - Other Topics

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ref (subject category)
art (subject category)

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By the author/editor: Samitas, Konstan ...; Malmhäll, Carina ...; Rådinger, Madele ...; Ramos-Ramírez, P ...; Lu, You, 1982; Deák, Tünde, 198 ...; show more...; Semitekolou, M.; Gaga, M.; Sjöstrand, Marga ...; Lötvall, Jan, 19 ...; Bossios, Apostol ...; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...

Articles in the publication: Plos One

By the university: University of Gothenburg; Karolinska Institutet

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