A method for 3D reconstruction of neuronal processes using semithin serial sections displayed as a cinematographic sequence.

Skoglund, Thomas, 1969 (författare): Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology

Pascher, R (författare)

Berthold, C H (författare)

(creator_code:org_t)

1995
1995
Engelska.
Ingår i: Journal of neuroscience methods. - 0165-0270. ; 61:1-2, s. 105-11

Abstract Ämnesord

Stäng

In order to study the organization and distribution of dendrites and axons in the cerebral cortex, we have developed a computer-assisted method for 3D reconstruction of neuronal processes based on serial light microscopic images displayed as a continuous sequence. A series of tangential sections (0.65 micron thick) through rat parietal cortex was aligned, digitized into the computer and then used to build a sequence (stack) of images which was stored to a digital real-time video disk. Apical dendrites located in dendritic bundles in laminae III and IV were traced through the sequence. Two tracing modes were tested: (1) cinematographic mode, in which the image stack was displayed continuously and automatically by the computer at various preset speeds (max. speed: 25 images/s) and (2) stepping mode, in which the interval between each image was varied manually according to the choice of the operator. Coordinates were stored in a database and used to build a 3D reconstruction where apical dendrites were displayed as wires or tubes. Tracing in cinematographic mode was about 3 times faster than tracing in stepping mode. We believe that the former mode exploits the built in 'filtering' capacity of the visual system to perform temporal averaging.

MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Neurovetenskaper

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