Sökning: id:"swepub:oai:gup.ub.gu.se/249979" > Overexpression of f...
Fältnamn | Indikatorer | Metadata |
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000 | 04150naa a2200601 4500 | |
001 | oai:gup.ub.gu.se/249979 | |
003 | SwePub | |
008 | 240528s2011 | |||||||||||000 ||eng| | |
024 | 7 | a https://gup.ub.gu.se/publication/2499792 URI |
024 | 7 | a https://doi.org/10.1371/journal.pone.00163322 DOI |
040 | a (SwePub)gu | |
041 | a eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Gasi, Delila,d 1982u Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences4 aut0 (Swepub:gu)xtandd |
245 | 1 0 | a Overexpression of full-length ETV1 transcripts in clinical prostate cancer due to gene translocation. |
264 | c 2011-01-26 | |
264 | 1 | b Public Library of Science (PLoS),c 2011 |
520 | a ETV1 is overexpressed in a subset of clinical prostate cancers as a fusion transcript with many different partners. However, ETV1 can also be overexpressed as a full-length transcript. Full-length ETV1 protein functions differently from truncated ETV1 produced by fusion genes. In this study we describe the genetic background of full-length ETV1 overexpression and the biological properties of different full-length ETV1 isoforms in prostate cancer. Break-apart FISH showed in five out of six patient samples with overexpression of full-length ETV1 a genomic rearrangement of the gene, indicating frequent translocation. We were able to study the rearrangements in more detail in two tumors. In the first tumor 5'-RACE on cDNA showed linkage of the complete ETV1 transcript to the first exon of a prostate-specific two exon ncRNA gene that maps on chromosome 14 (EST14). This resulted in the expression of both full-length ETV1 transcripts and EST14-ETV1 fusion transcripts. In chromosome spreads of a xenograft derived from the second prostate cancer we observed a complex ETV1 translocation involving a chromosome 7 fragment that harbors ETV1 and fragments of chromosomes 4 and 10. Further studies revealed the overexpression of several different full-length transcripts, giving rise to four protein isoforms with different N-terminal regions. Even the shortest isoform synthesized by full-length ETV1 stimulated in vitro anchorage-independent growth of PNT2C2 prostate cells. This contrasts the lack of activity of even shorter N-truncated ETV1 produced by fusion transcripts. Our findings that in clinical prostate cancer overexpression of full-length ETV1 is due to genomic rearrangements involving different chromosomes and the identification of a shortened biologically active ETV1 isoform are highly relevant for understanding the mechanism of ETV1 function in prostate cancer. | |
650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicin0 (SwePub)3022 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicine0 (SwePub)3022 hsv//eng |
653 | a Chromosomes | |
653 | a Human | |
653 | a DNA-Binding Proteins | |
653 | a genetics | |
653 | a Gene Expression Regulation | |
653 | a Neoplastic | |
653 | a Humans | |
653 | a Male | |
653 | a Prostatic Neoplasms | |
653 | a genetics | |
653 | a Protein Isoforms | |
653 | a RNA | |
653 | a Neoplasm | |
653 | a analysis | |
653 | a Transcription Factors | |
653 | a genetics | |
653 | a Translocation | |
653 | a Genetic | |
700 | 1 | a van der Korput, Hetty A4 aut |
700 | 1 | a Douben, Hannie C4 aut |
700 | 1 | a de Klein, Annelies4 aut |
700 | 1 | a de Ridder, Corrina M4 aut |
700 | 1 | a van Weerden, Wytske M4 aut |
700 | 1 | a Trapman, Jan4 aut |
710 | 2 | a Göteborgs universitetb Institutionen för kliniska vetenskaper4 org |
773 | 0 | t PloS oned : Public Library of Science (PLoS)g 6:1q 6:1x 1932-6203 |
856 | 4 | u https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0016332&type=printable |
856 | 4 8 | u https://gup.ub.gu.se/publication/249979 |
856 | 4 8 | u https://doi.org/10.1371/journal.pone.0016332 |
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